Barra Y, de Lapeyrière O, Planche J
J Natl Cancer Inst. 1979 Dec;63(6):1441-5.
Tumor-specific transplantation antigen (TSTA) and polyomavirus-induced tumor-associated surface antigen (TASA) were isolated from polyomavirus-transformed BALB/c mouse cells with the use of 3-M KCl solubilization and affinity chromatography on antibody-coated Sepharose 4B. Particular conditions were chosen for removal of the nonspecifically bound proteins from the gel. Under these conditions, the ratio between the amounts of crude extract and specifically bound proteins giving the same TASA activity was about 5,000. Mice inoculated with this material were protected against tumor challenge. We therefore assume that TSTA is a part of TASA. Furthermore, these antigens can be dissociated from the major histocompatibility antigens.
利用3M KCl溶解和在抗体包被的琼脂糖4B上进行亲和层析，从多瘤病毒转化的BALB/c小鼠细胞中分离出肿瘤特异性移植抗原（TSTA）和多瘤病毒诱导的肿瘤相关表面抗原（TASA）。选择了特定条件以从凝胶中去除非特异性结合的蛋白质。在这些条件下，产生相同TASA活性的粗提物与特异性结合蛋白的量之比约为5000。接种这种物质的小鼠可免受肿瘤攻击。因此，我们假设TSTA是TASA的一部分。此外，这些抗原可以与主要组织相容性抗原解离。
