通过抑制短暂性脑缺血中增加的羟基自由基形成来预防延迟性神经元死亡。
Delayed neuronal death prevented by inhibition of increased hydroxyl radical formation in a transient cerebral ischemia.
作者信息
Yamamoto T, Yuki S, Watanabe T, Mitsuka M, Saito K I, Kogure K
机构信息
Pharmaceuticals Laboratory I, Yokohama Research Center, Mitsubishi Chemical Corp., Aoba-ku, Yokohama, Japan.
出版信息
Brain Res. 1997 Jul 11;762(1-2):240-2. doi: 10.1016/s0006-8993(97)00490-3.
The salicylate-trapping method was used to detect hydroxyl radicals by measurement of stable adduct dihydroxybenzoic acid (DHBA). Ten minutes of forebrain ischemia followed by reperfusion induced the increase in DHBA in rat hippocampal perfusates. Postischemic treatment with a free radical scavenger, 3-methyl-1-phenyl-2-pyrazolin-5-one (MCI-186), significantly reduced the increase in DHBA and suppressed delayed neuronal death in the hippocampal CA1 region.
采用水杨酸盐捕获法，通过测量稳定加合物二羟基苯甲酸（DHBA）来检测羟自由基。大鼠前脑缺血10分钟后再灌注，可导致海马灌注液中DHBA增加。用自由基清除剂3-甲基-1-苯基-2-吡唑啉-5-酮（MCI-186）进行缺血后处理，可显著降低DHBA的增加，并抑制海马CA1区的迟发性神经元死亡。
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