Usherwood E J, Brooks J W, Sarawar S R, Cardin R D, Young W D, Allen D J, Doherty P C, Nash A A
Department of Veterinary Pathology, University of Edinburgh, Summerhall, UK.
J Gen Virol. 1997 Aug;78 ( Pt 8):2025-30. doi: 10.1099/0022-1317-78-8-2025.
Perforin-mediated cytotoxic T cell killing has been suggested to be of importance in the control of noncytopathic virus infections, based on studies with lymphocytic choriomeningitis virus (LCMV). We examined the role of perforin in a mouse model of gammaherpesvirus infection using transgenic perforin-deficient mice. Previous work from this laboratory has shown that CD8 T cells are essential for the resolution of the acute lung infection and control of latently infected B cells in murine gamma-herpesvirus 68 infection. The absence of perforin did not significantly affect the kinetics of either the lytic lung infection or the latent spleen infection. Lymphocytes from both perforin-deficient and control mice secreted comparable levels of IFN-gamma, IL-10 and IL-6. In addition, lymphocytes from both strains had similar levels of CD3epsilon-dependent cytotoxic activity in the spleen, draining lymph nodes and bronchoalveolar lavage. These data indicate that the lack of perforin has little affect on the ability of mice to control an experimental gammaherpesvirus infection.
基于对淋巴细胞性脉络丛脑膜炎病毒（LCMV）的研究，穿孔素介导的细胞毒性T细胞杀伤作用被认为在控制非细胞病变性病毒感染中具有重要意义。我们使用转基因穿孔素缺陷小鼠，在γ疱疹病毒感染的小鼠模型中研究了穿孔素的作用。该实验室之前的工作表明，在鼠γ疱疹病毒68感染中，CD8 T细胞对于急性肺部感染的消退和潜伏感染B细胞的控制至关重要。穿孔素的缺失并未显著影响溶细胞性肺部感染或潜伏性脾脏感染的动力学。穿孔素缺陷小鼠和对照小鼠的淋巴细胞分泌的IFN-γ、IL-10和IL-6水平相当。此外，两种品系的淋巴细胞在脾脏、引流淋巴结和支气管肺泡灌洗中具有相似水平的CD3ε依赖性细胞毒性活性。这些数据表明，穿孔素的缺乏对小鼠控制实验性γ疱疹病毒感染的能力影响很小。
