Reimer R A, Field C J, McBurney M I
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
Diabetologia. 1997 Aug;40(8):871-8. doi: 10.1007/s001250050762.
Weaning onto chow diets causes the highest incidence of diabetes in the BB rat. Changes in gut development and absorption of nutrients in the diabetes prone rat and the subsequent effect on pancreatic function may play a role in the ultimate development of the disease. BB diabetes prone (dp) and BB normal (n) dams were fed chow diets. Pups were killed at various ages ranging from 7 to 30 days. BBdp rats had higher small intestine and colon weights expressed per body weight at all ages (p < 0.0001). RNA content (mg/g) in the jejunum, ileum and colon was higher in the BBdp rats beginning at the critical period at 21 days and maintained at 24 days and 30 days (p < 0.0001). Proglucagon message decreased with age in both BBdp and BBn animals (p < 0.0001). Levels of proglucagon mRNA were higher in BBdp compared to BBn animals only in the ileum at 10 days (p < 0.01). Adjusting for total ileal and colonic RNA content resulted in BBdp animals having higher total colonic proglucagon mRNA at 21, 24 and 30 days (p < 0.0001). Plasma GLP-1(7-36) amide was more than doubled in BBdp compared to BBn animals (p < 0.0005) at 30 days. Expressing sodium-dependent D-glucose co-transporter (SGLT-1), GLUT2 and GLUT5 mRNA per total jejunal RNA shows increased transporter mRNA in BBdp compared to BBn rats at weaning (21 days) (p < 0.05). Radical differences exist between BBdp and BBn animals at 'critical periods' in both proglucagon and glucose transporter gene expression. These differences may help explain altered growth and diseases incidence between these two strains.
断奶后改用普通饲料喂养会使BB大鼠患糖尿病的几率最高。糖尿病易感大鼠肠道发育和营养吸收的变化以及随后对胰腺功能的影响可能在该疾病的最终发展中起作用。给BB糖尿病易感（dp）和BB正常（n）的母鼠喂食普通饲料。在7至30天的不同年龄段处死幼崽。BBdp大鼠在所有年龄段的小肠和结肠重量与体重的比值均较高（p < 0.0001）。从21天的关键期开始，BBdp大鼠空肠、回肠和结肠中的RNA含量（mg/g）升高，并在24天和30天保持升高（p < 0.0001）。在BBdp和BBn动物中，胰高血糖素原信息均随年龄下降（p < 0.0001）。仅在10天时，BBdp动物回肠中的胰高血糖素原mRNA水平高于BBn动物（p < 0.01）。校正回肠和结肠总RNA含量后，BBdp动物在21、24和30天时结肠中胰高血糖素原mRNA总量更高（p < 0.0001）。在30天时，与BBn动物相比，BBdp动物血浆GLP-1(7-36)酰胺增加了一倍多（p < 0.0005）。以空肠总RNA为参照来表示钠依赖性D-葡萄糖共转运蛋白（SGLT-1）、GLUT2和GLUT5 mRNA，结果显示断奶时（21天）BBdp大鼠的转运蛋白mRNA比BBn大鼠增加（p < 0.05）。在胰高血糖素原和葡萄糖转运蛋白基因表达的“关键期”，BBdp和BBn动物之间存在显著差异。这些差异可能有助于解释这两种品系之间生长和疾病发生率的改变。
