[Experimental study of immunity in anti-plasmodium infection on preserved spleen and splenectomized mice].

OBJECTIVE

To investigate the function of the spleen as well as the changes of splenic function after hemisplenectomy and hemisplenic autotransplanted in plasmodium yoelili infected mice (first and second infection).

METHODS

336 mice were divided into six groups: 1. total splenectomy, 2. hemisplenectomy, 3. hemisplenic autotransplanted, 4. sham-operation, 5. total splenectomy of 2nd infection, 6. sham-operation of the 2nd infection. 3 months after operation (1-4) or 15 day after 1st infection and operation (5-6) in mice intraperitoneal inoculations were initiated.

RESULTS

1. First infection: in total splenectomy group the antiplasmodium antibody reduced, the remanent rate of 51Cr-RBC in circulation was high, RBC count continously decreased, parasitized erytrocytes still were seen after 1 month. These changes were mild in the preserved spleen. But the production of antiplasmodium antibody and spleen uptake rate of 51Cr-RBC of the hemisplenic autotrans aplaned were rather lower than those of the shamoperate group. 2. Second lethal yoelli infection: in the mice of shamoperation of 2nd yoelii infection the parasitized erytrocyte was lower than that of shamoperated group, but the higher level antiplasmodium antibody and uptake rates of 51Cr-RBC of unit weight splenic tissue.

CONCLUSIONS

Under the plasmodium infection preserved hemispleen alternate normal spleen to play the immunologic role, splenic tissue autotransplantation can fairly restore the partial function of spleen. Different preserved spleen method is a useful operation for clinic.