来自海栖热袍菌的一种耐热多结构域木聚糖酶的克隆与序列分析
Cloning and sequence of a thermostable multidomain xylanase from the bacterium Rhodothermus marinus.
作者信息
Nordberg Karlsson E, Bartonek-Roxå E, Holst O
机构信息
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Sweden.
出版信息
Biochim Biophys Acta. 1997 Aug 7;1353(2):118-24. doi: 10.1016/s0167-4781(97)00093-6.
The gene (xyn1) encoding a Rhodothermus marinus xylanase has been cloned and expressed in Escherichia coli. The gene comprises 5 different domains in an unusual combination. The cellulose binding domains (CBDs) encoded by xyn1 are repeated in tandem at the N-terminus and show similarity with the CBD family IV. The xyn1-gene is the first example encoding a CBD family IV in combination with a xylan hydrolyzing catalytic domain of the glycosyl hydrolase family 10.
编码海栖热袍菌木聚糖酶的基因（xyn1）已在大肠杆菌中克隆并表达。该基因以一种不同寻常的组合包含5个不同的结构域。由xyn1编码的纤维素结合结构域（CBDs）在N端串联重复，与IV型CBD家族具有相似性。xyn1基因是第一个编码与糖基水解酶家族10的木聚糖水解催化结构域相结合的IV型CBD家族的例子。
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