Inhibitory monoclonal antibodies to human cytochrome P450 2D6.
作者信息
Krausz K W, Yang T J, Gonzalez F J, Shou M, Gelboin H V
机构信息
Laboratory of Molecular Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, U.S.A.
出版信息
Biochem Pharmacol. 1997 Jul 1;54(1):15-7. doi: 10.1016/s0006-2952(97)00261-x.
PMID:9296346
Abstract
Two monoclonal antibodies (MAbs) have been isolated that bind to human P450 2D6 and inhibit 2D6 catalyzed bufuralol 1-hydroxylation by 90%. One but not both of the MAbs immunoblotted 2D6. The MAbs were highly specific to 2D6 and did not cross-react with other P450s. Inhibitory monoclonal antibodies will be useful for determining the contribution of 2D6 to the metabolism of a wide variety of 2D6 and other P450 substrates in human tissues containing multiple P450s.
摘要
Zotero 插件