采用柱切换高效液相色谱-电喷雾质谱法对血浆样本中的沙丁胺醇进行快速药代动力学筛选。
Rapid pharmacokinetic screening of salbutamol in plasma samples by column-switching high-performance liquid chromatography-electrospray mass spectrometry.
作者信息
Schmeer K, Sauter T, Schmid J
机构信息
Department of Pharmacokinetics and Metabolism, Dr. Karl Thomae GmbH, Riss, Germany.
出版信息
J Chromatogr A. 1997 Aug 8;777(1):67-72. doi: 10.1016/s0021-9673(97)00432-9.
In order to obtain pharmacokinetic data from studies in humans, a sensitive and selective assay for the quantification of salbutamol in human plasma samples was required. This report describes an automated high-performance liquid chromatography-mass spectrometry assay with pre-column enrichment using internal standard calibration for the quantification of salbutamol and the validation of the assay. The lower limit of quantitation is 0.2 ng/ml with an accuracy and imprecision of less than 7%. The analysis time is 8 min per sample.
为了从人体研究中获取药代动力学数据，需要一种灵敏且选择性强的方法来定量测定人血浆样本中的沙丁胺醇。本报告描述了一种采用柱前富集、内标校准的自动化高效液相色谱-质谱分析法，用于定量测定沙丁胺醇并对该分析方法进行验证。定量下限为0.2 ng/ml，准确度和不精密度均小于7%。每个样本的分析时间为8分钟。
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