用高效液相色谱法测定人血浆中左旋多巴/左旋酪氨酸比值。作为转移性恶性黑色素瘤标志物的实用性。

Determination of the L-DOPA/L-tyrosine ratio in human plasma by high-performance liquid chromatography. Usefulness as a marker in metastatic malignant melanoma.

作者信息

Letellier S, Garnier J P, Spy J, Bousquet B

机构信息

Laboratoire de Biochimie A et de Neurobiologie, Hôpital Saint Louis, Paris, France.

出版信息

J Chromatogr B Biomed Sci Appl. 1997 Aug 15;696(1):9-17. doi: 10.1016/s0378-4347(97)00206-5.

DOI:10.1016/s0378-4347(97)00206-5

PMID:9300903

Abstract

A first procedure was devised for determining 3,4-dihydroxyphenylalanine (L-DOPA) in human plasma by isocratic RP-HPLC coupled with electrochemical detection. A second procedure was devised for determining 3-hydroxyphenylalanine (L-tyrosine) in human plasma by isocratic RP-HPLC coupled with fluorescence detection. These methods were used to ascertain the L-DOPA/L-tyrosine ratio in plasma of patients with melanoma. Reference values were established by analysis of the L-DOPA/L-tyrosine ratio in the plasma of 35 normal healthy subjects. For 29 patients diagnosed as having melanoma without metastasis, the L-DOPA/L-tyrosine (11.96 x 10(-5) +/- 2.69 x 10(-5)) level was not significantly different from that of 35 normal controls (11.20 x 10(-5) +/- 2.92 x 10(-5)). However, this level was significantly increased (p < 0.05) in the plasma of 17 patients with developing metastasis (21.02 x 10(-5) +/- 4.68 x 10(-5)).

摘要

设计了一种通过等度反相高效液相色谱法结合电化学检测来测定人血浆中3,4-二羟基苯丙氨酸（L-多巴）的方法。还设计了一种通过等度反相高效液相色谱法结合荧光检测来测定人血浆中3-羟基苯丙氨酸（L-酪氨酸）的方法。这些方法用于确定黑色素瘤患者血浆中的L-多巴/L-酪氨酸比值。通过分析35名正常健康受试者血浆中的L-多巴/L-酪氨酸比值建立了参考值。对于29名被诊断为无转移黑色素瘤的患者，其L-多巴/L-酪氨酸水平（11.96×10⁻⁵±2.69×10⁻⁵）与35名正常对照者（11.20×10⁻⁵±2.92×10⁻⁵）相比无显著差异。然而，在17名发生转移的患者血浆中，该水平显著升高（p<0.05）（21.02×10⁻⁵±4.68×10⁻⁵）。