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[金黄色葡萄球菌菌株对无机硫源的利用]

[Utilization of inorganic sulfur sources by Staphylococcus aureus strains].

作者信息

Seltmann G, Voigt W

出版信息

Z Allg Mikrobiol. 1977;17(6):437-50. doi: 10.1002/jobm.3630170605.

Abstract

Staphylococcus aureus strains of different host-adapted variants (Meyer 1966) have been tested for their ability to use inorganic sulfur sources. All the 25 strains tested were able to utilize sodium sulfide as sulfur source in a medium similar to that described by Kloos and Pattee (1965). Using S. aureus strain 116/74 grown in a medium containing Na2-35S as the only sulfur source we studied incorporation and insertion of inorganic sulfide into sulfur containing amino acids. In disintegrated and fractionated cellular material we could find 35S labelled homocystine and methionine as major compounds, and cystine, cysteic acid, homocysteic acid, and beta-sulphopyruvate as minor compounds. The occurrence of homocystine and the sulfonic acids in bacterial proteins is rather uncommon.

摘要

已对不同宿主适应变体的金黄色葡萄球菌菌株(Meyer,1966年)利用无机硫源的能力进行了测试。所测试的25株菌株均能够在类似于Kloos和Pattee(1965年)所描述的培养基中利用硫化钠作为硫源。使用在含有Na2-35S作为唯一硫源的培养基中生长的金黄色葡萄球菌菌株116/74,我们研究了无机硫化物掺入和插入含硫氨基酸的情况。在破碎和分级分离的细胞物质中,我们发现35S标记的高胱氨酸和蛋氨酸是主要化合物,而胱氨酸、半胱氨酸、高半胱氨酸和β-磺基丙酮酸是次要化合物。高胱氨酸和磺酸在细菌蛋白质中的出现相当罕见。

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