用于细菌感染诊断的真细菌方法。
Eubacterial approach to the diagnosis of bacterial infection.
作者信息
Ley B E, Linton C J, Longhurst S, Jalal H, Millar M R
机构信息
Bristol Royal Hospital for Sick Children, Department of Paediatric Oncology.
出版信息
Arch Dis Child. 1997 Aug;77(2):148-9. doi: 10.1136/adc.77.2.148.
Abstract
Computed tomography revealed a frontal empyema in an 8 month old boy who presented with a prolonged convulsion. Before admission he had received oral antibiotics and all specimens were negative on culture. The presence of bacteria in cerebrospinal and empyema fluid was demonstrated using the polymerase chain reaction (PCR) with 16S rRNA gene primers. A presumptive identification of Streptococcus pneumoniae was made by comparison of PCR products with those derived from known bacteria using denaturing gradient gel electrophoresis.
摘要
计算机断层扫描显示，一名8个月大出现长时间惊厥的男童患有额部积脓。入院前他接受了口服抗生素治疗，所有标本培养结果均为阴性。使用针对16S rRNA基因的引物，通过聚合酶链反应（PCR）在脑脊液和积脓液中检测到了细菌。通过变性梯度凝胶电泳将PCR产物与已知细菌的产物进行比较，初步鉴定为肺炎链球菌。
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本文引用的文献
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