Bennett K D, Bondareff W
Am J Anat. 1977 Sep;150(1):175-84. doi: 10.1002/aja.1001500112.
Neurons isolated from the lateral vestibular nucleus of young adult and senescent Fischer-344 rats were incubated with fluorescamine-labelled Concanavalin A (fl-Con A) alone, or following incubation in trypsin or Vibrio cholerae neuraminidase. They were then observed and photographed. Microdensitometric analysis of fluorescence micrographs showed that senescent rat neurons were significantly more fluorescent than those from young adult rats. Additionally, either patches or caps of fl-Con A were seen on the surface of neurons from senescent rats, while most young adult rat neurons bound fl-Con A uniformly. Pretreatment with trypsin or neuraminidase had no effect on the amount of fluorescence on the surface of senescent rat neurons, and only a slight effect on the surface distribution. Trypsin and neuronal plasma membranes of young adult rats and a rearrangement of the binding pattern in the majority of neurons observed.
将从年轻成年和衰老的Fischer-344大鼠的外侧前庭核分离出的神经元，单独用荧光胺标记的伴刀豆球蛋白A（fl-Con A）孵育，或在胰蛋白酶或霍乱弧菌神经氨酸酶中孵育后再进行孵育。然后对它们进行观察和拍照。荧光显微镜照片的显微密度分析表明，衰老大鼠的神经元比年轻成年大鼠的神经元荧光明显更强。此外，在衰老大鼠神经元的表面可见fl-Con A的斑块或帽状结构，而大多数年轻成年大鼠神经元则均匀地结合fl-Con A。用胰蛋白酶或神经氨酸酶预处理对衰老大鼠神经元表面的荧光量没有影响，对表面分布只有轻微影响。观察到胰蛋白酶和年轻成年大鼠的神经元质膜以及大多数神经元结合模式的重排。
