两个发夹结构作为鼠白血病病毒病毒粒子中核心RNA包装信号的作用。
A role for two hairpin structures as a core RNA encapsidation signal in murine leukemia virus virions.
作者信息
Mougel M, Barklis E
机构信息
Vollum Institute for Advanced Biomedical Research and Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201-3098, USA.
出版信息
J Virol. 1997 Oct;71(10):8061-5. doi: 10.1128/JVI.71.10.8061-8065.1997.
Abstract
Four putative hairpin structures (hairpins A to D) are involved in the specific encapsidation of Moloney murine leukemia virus (M-MuLV) RNA into M-MuLV virus particles. The C and D elements, encompassing M-MuLV viral nucleotides 310 to 374, facilitate encapsidation of heterologous RNA into virions. Thus, these two elements appear to act as a core RNA encapsidation signal. The loop sequences of the putative C and D hairpins are identical (GACG). However, when GACG loops were introduced into RNAs on heterologous stem sequences, they increased encapsidation levels only three- to fourfold. These results suggest that C and D stem-and-loop sequences contribute to the M-MuLV cis-acting site for encapsidation.
摘要
四个假定的发夹结构（发夹A至D）参与莫洛尼鼠白血病病毒（M-MuLV）RNA特异性包装进M-MuLV病毒颗粒的过程。包含M-MuLV病毒核苷酸310至374的C和D元件促进异源RNA包装进病毒粒子。因此，这两个元件似乎作为核心RNA包装信号发挥作用。假定的C和D发夹的环序列相同（GACG）。然而，当将GACG环引入异源茎序列的RNA时，它们仅将包装水平提高了三到四倍。这些结果表明，C和D茎环序列有助于M-MuLV顺式作用包装位点。
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