[通过中性磷脂脂质体将功能基因转移到真核细胞中]
[Transfer of the functional genes into eucaryotic cells by neutral phospholipid liposomes].
作者信息
Zhdanov R I, Buneeva O A, Podobed O V, Kutsenko N G, Tsvetkova T A, Lavrenova T P
出版信息
Vopr Med Khim. 1997 Jul-Aug;43(4):212-6.
Transfection of plasmid DNAs containing b-galactosidase gene (pQE-LacZ) or alkaline phosphatase (pCSEAP) into L929 cell line using was studied. The complexes between plasmid DNA and liposomes containing Ca ions and glycyrrhizic acid or &-tocopherol caused successful transfection of functional genes into L929 cells. The efficiency of transfection of plasmid DNAs into L929 cells using polynucleotide-metallo(II)-liposome complexes were 30-50% from the efficiency value of calcium phosphate coprecipitation transfection.
研究了使用含有β-半乳糖苷酶基因的质粒DNA（pQE-LacZ）或碱性磷酸酶（pCSEAP）转染L929细胞系。质粒DNA与含有钙离子和甘草酸或α-生育酚的脂质体之间的复合物成功地将功能基因转染到L929细胞中。使用多核苷酸-金属（II）-脂质体复合物将质粒DNA转染到L929细胞中的效率相对于磷酸钙共沉淀转染的效率值为30%-50%。
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