Kim K K, Yokota H, Kim R, Kim S H
Structural Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Protein Sci. 1997 Oct;6(10):2268-70. doi: 10.1002/pro.5560061023.
A gene coding for a protein homologous to a translation initiation factor of eukaryotes, eIF5A, was cloned from Methanococcus jannaschii, a hyperthermophile with an optimum growth temperature of 85 degrees C. The protein was overexpressed, purified and crystallized. The crystals were obtained by vapor diffusion method with 8% PEG 4000 as precipitant and belong to space group P4(1)22 with unit cell dimensions a = b = 45.52 A and c = 155.59 A. These crystals diffract to at least 2.2 A resolution.
从最适生长温度为85摄氏度的嗜热栖热菌詹氏甲烷球菌中克隆出了一个编码与真核生物翻译起始因子eIF5A同源蛋白质的基因。该蛋白质被过量表达、纯化并结晶。晶体通过气相扩散法获得,以8%聚乙二醇4000作为沉淀剂,属于空间群P4(1)22,晶胞参数为a = b = 45.52 Å,c = 155.59 Å。这些晶体的衍射分辨率至少为2.2 Å。