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在酿酒酵母中,从COX1前体mRNA中有效切除内含子aI5β并不需要线粒体蛋白质合成。

Mitochondrial protein synthesis is not required for efficient excision of intron aI5 beta from COX1 pre-mRNA in Saccharomyces cerevisiae.

作者信息

Johnson C H, McEwen J E

机构信息

Geriatrics Research and Education Clinical Center, John L. McClellan Memorial Veterans Hospital, Little Rock, AR 72205, USA.

出版信息

Mol Gen Genet. 1997 Sep;256(1):88-91. doi: 10.1007/s004380050549.

Abstract

Splicing of the group I intron aI5 beta from the yeast mitochondrial COX1 transcript requires at least four proteins, encoded by the nuclear genes PET54, MRS1/PET157, SUV3 and MSS18. These proteins either act directly to facilitate intron aI5 beta excision, or indirectly in some manner. One possible indirect mode of action of these nuclear gene products is in stimulation of expression of a mitochondrial protein, such as a maturase, that is necessary for intron aI5 beta excision. To test this possibility, splicing of intron aI5 beta was examined in a rho-strain, which is incapable of mitochondrial protein synthesis. A quantitative RT-PCR assay was set up to compare levels of spliced COX1 mRNA present in three strains: a wild-type rho + strain; the rho-strain 7-49b-11, which retains the entire COX1 transcription unit; and a strain bearing a null mutation in the nuclear PET54 gene. The results showed that excision of aI5 beta occurs relatively efficiently in the rho-strain, and therefore does not require any mitochondrial-encoded proteins.

摘要

酵母线粒体COX1转录本中I类内含子aI5β的剪接至少需要四种由核基因PET54、MRS1/PET157、SUV3和MSS18编码的蛋白质。这些蛋白质要么直接作用以促进内含子aI5β的切除,要么以某种间接方式起作用。这些核基因产物一种可能的间接作用模式是刺激线粒体蛋白质(如成熟酶)的表达,而成熟酶是内含子aI5β切除所必需的。为了验证这种可能性,在一个不能进行线粒体蛋白质合成的rho-菌株中检测了内含子aI5β的剪接。建立了定量RT-PCR测定法,以比较三种菌株中存在的剪接COX1 mRNA水平:野生型rho+菌株;保留整个COX1转录单元的rho-菌株7-49b-11;以及在核PET54基因中携带无效突变的菌株。结果表明,aI5β在rho-菌株中的切除相对高效,因此不需要任何线粒体编码的蛋白质。

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