Poulin M L, Botelho M J, Chiu I M
Department of Internal Medicine, Ohio State University, Davis Medical Research Center, Columbus 43210, USA.
Mol Cell Biochem. 1997 Oct;175(1-2):11-9. doi: 10.1023/a:1006855524371.
We report the nucleotide sequences of two fibroblast growth factor receptor (FGFR) cDNAs, FGFR1 and FGFR3, from the newt species Notophthalmus viridescens. These two cDNA sequences and a previously published newt FGFR cDNA, FGFR2, were used to derive the amino acid sequences which were then compared with their homologues from other species. This comparison shows that the intracellular tyrosine kinase domain is highly conserved across the species examined with the second half of the domain slightly more conserved than the first half. The 3' portion of the carboxyl terminal tail is not very highly conserved. The comparison of the extracellular portion of FGFR2 shows a high degree of conservation among the Ig-like domains and a low degree of conservation in the region that links the third Ig-like domain with the transmembrane domain.
我们报道了来自美西螈（Notophthalmus viridescens）的两个成纤维细胞生长因子受体（FGFR）cDNA，即FGFR1和FGFR3的核苷酸序列。利用这两个cDNA序列以及先前发表的美西螈FGFR cDNA即FGFR2推导氨基酸序列，然后将其与其他物种的同源物进行比较。该比较表明，在所研究的物种中，细胞内酪氨酸激酶结构域高度保守，该结构域的后半部分比前半部分略为保守。羧基末端尾巴的3'部分保守性不是很高。FGFR2细胞外部分的比较显示，免疫球蛋白样结构域之间具有高度保守性，而在连接第三个免疫球蛋白样结构域与跨膜结构域的区域保守性较低。
