酵母中[URE3]的朊病毒模型：自发产生及传播所需条件。
The prion model for [URE3] of yeast: spontaneous generation and requirements for propagation.
作者信息
Masison D C, Maddelein M L, Wickner R B
机构信息
Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, Building 8, Room 225, National Institutes of Health, 8 Center Drive MSC0830, Bethesda, MD 20892-0830, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Nov 11;94(23):12503-8. doi: 10.1073/pnas.94.23.12503.
The genetic properties of the non-Mendelian element, [URE3], suggest that it is a prion (infectious protein) form of Ure2p, a mediator of nitrogen regulation in Saccharomyces cerevisiae. Into a ure2Delta strain (necessarily lacking [URE3]), we introduced a plasmid overproducing Ure2p. This induced the frequent "spontaneous generation" of [URE3], with properties identical to the original [URE3]. Altering the translational frame only in the prion-inducing domain of URE2 shows that it is Ure2 protein (and not URE2 RNA) that induces appearance of [URE3]. The proteinase K-resistance of Ure2p is unique to [URE3] strains and is not seen in nitrogen regulation of normal strains. The prion-inducing domain of Ure2p (residues 1-65) can propagate [URE3] in the absence of the C-terminal part of the molecule. In contrast, the C-terminal part of Ure2p cannot be converted to the prion (inactive) form without the prion-inducing domain covalently attached. These experiments support the prion model for [URE3] and extend our understanding of its propagation.
非孟德尔遗传因子[URE3]的遗传特性表明，它是酿酒酵母中氮调节介质Ure2p的一种朊病毒（感染性蛋白质）形式。在一个ure2Δ菌株（必然缺乏[URE3]）中，我们导入了一个过量表达Ure2p的质粒。这导致了[URE3]的频繁“自发产生”，其特性与原始的[URE3]相同。仅在URE2的朊病毒诱导结构域中改变翻译框架表明，诱导[URE3]出现的是Ure2蛋白（而非URE2 RNA）。Ure2p对蛋白酶K的抗性是[URE3]菌株所特有的，在正常菌株的氮调节中未观察到。Ure2p的朊病毒诱导结构域（第1至65位氨基酸残基）在没有分子C端部分的情况下也能传播[URE3]。相反，Ure2p的C端部分在没有共价连接的朊病毒诱导结构域的情况下不能转化为朊病毒（无活性）形式。这些实验支持了[URE3]的朊病毒模型，并扩展了我们对其传播的理解。
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