Fujii H, Inagaki M, Kasai S, Miyokawa N, Tokusashi Y, Gabrielson E, Hruban R H
Department of Pathology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287, USA.
Am J Pathol. 1997 Nov;151(5):1447-54.
Intraductal papillary-mucinous neoplasms (IPMNs) of the pancreas are ideal neoplasms to study clonal progression and genetic diversity because of their large size and prominent intraductal component. We microdissected 55 histologically defined areas from 13 IPMNs, extracted the DNA from each, and performed polymerase chain reaction (PCR)-based microsatellite analysis to detect loss of heterozygosity on chromosome arms 1p, 3p, 6q, 8p, 9p, 17p, 18q, and 22q. LOH was identified at 1p in two cases, at 3p in four cases, at 6q in seven cases, at 8p in four cases, at 9p in eight cases, at 17p in five cases, at 18q in five cases, and at 22q in one of the IPMNs examined. In one of the IPMNs, the allelic losses were uniform throughout multiple microdissected areas, and in four of the IPMNs, there was evidence of clonal progression. In contrast, in three of the IPMNs, substantial allelic heterogeneity was seen. This remarkable heterogeneity may, in part, be due to the slow growth rate of these neoplasms.
胰腺导管内乳头状黏液性肿瘤（IPMNs）因其体积大且导管内成分突出，是研究克隆进展和基因多样性的理想肿瘤。我们从13例IPMNs中显微切割了55个组织学定义的区域，从每个区域提取DNA，并进行基于聚合酶链反应（PCR）的微卫星分析，以检测1p、3p、6q、8p、9p、17p、18q和22q染色体臂上的杂合性缺失。在2例中检测到1p杂合性缺失，4例检测到3p杂合性缺失，7例检测到6q杂合性缺失，4例检测到8p杂合性缺失，8例检测到9p杂合性缺失，5例检测到17p杂合性缺失，5例检测到18q杂合性缺失，在所检查的1例IPMN中检测到22q杂合性缺失。在1例IPMN中，多个显微切割区域的等位基因缺失是一致的，在4例IPMN中，有克隆进展的证据。相反，在3例IPMN中，观察到明显的等位基因异质性。这种显著的异质性可能部分归因于这些肿瘤的生长速度缓慢。
