Abe K, Kato M, Saito H
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, The University of Tokyo, Japan.
Neurosci Res. 1997 Oct;29(2):129-34. doi: 10.1016/s0168-0102(97)00081-3.
An amyloid-binding dye Congo red has been reported to prevent the neurotoxic effect of Alzheimer's amyloid beta protein (Abeta). In the present study, we investigated the effect of Congo red in cultured rat cortical astrocytes. Abeta (1 nM-10 microM) did not cause cell death, but potently inhibited the cellular redox activity as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. Congo red (0.2-20 microM), when added together with or prior to Abeta, significantly blocked Abeta-induced inhibition of redox activity. Furthermore, when Congo red was added after treatment with Abeta, the inhibited redox activity was restored to normal, indicating that Congo red can reverse Abeta-induced cellular stress. The reversing effect of Congo red cannot be explained by the inhibition of Abeta fibril formation and suggests a novel aspect of the interaction of Congo red with Abeta.
据报道，一种淀粉样蛋白结合染料刚果红可预防阿尔茨海默病淀粉样β蛋白（Aβ）的神经毒性作用。在本研究中，我们调查了刚果红对培养的大鼠皮质星形胶质细胞的影响。Aβ（1 nM - 10 μM）不会导致细胞死亡，但通过3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐（MTT）还原试验测定，其能有效抑制细胞氧化还原活性。当与Aβ一起添加或在Aβ之前添加时，刚果红（0.2 - 20 μM）可显著阻断Aβ诱导的氧化还原活性抑制。此外，在用Aβ处理后添加刚果红时，被抑制的氧化还原活性恢复正常，这表明刚果红可逆转Aβ诱导的细胞应激。刚果红的这种逆转作用无法通过抑制Aβ纤维形成来解释，这提示了刚果红与Aβ相互作用的一个新方面。
