Shangkuan Y H, Tsao C M, Lin H C
Division of Bacteriology, Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan, Republic of China.
J Med Microbiol. 1997 Nov;46(11):941-8. doi: 10.1099/00222615-46-11-941.
The rRNA gene restriction patterns and the polymerase chain reaction (PCR) fingerprinting types of 53 Vibrio cholerae O1 isolates were studied. Five and eight patterns were observed from 27 toxigenic and 26 non-toxigenic O1 isolates after BglI cleavage. PCR fingerprinting with three primer sets aimed at enterobacterial repetitive intergenic consensus (ERIC) sequences, ERIC-related sequences in V. cholerae, another kind of repeated sequences in V. cholerae (VCR) and arbitrary sequences divided the same strains into seven and 10 PCR types, respectively. Eight ribotypes had unique PCR patterns. PCR fingerprinting identified more than one pattern among isolates within each of the remaining ribotypes. However, ribotyping was able to differentiate the same PCR types in one case. A single ribotype and a single PCR pattern were found in toxigenic O1 strains isolated in Taiwan from imported food and imported cases of cholera between 1993 and 1995. Typing of V. cholerae O1 by PCR fingerprinting correlated well with ribotyping, but was more discriminating. PCR assay provides a rapid and simple means of typing these strains for epidemiological studies.
对53株霍乱弧菌O1分离株的rRNA基因限制性酶切图谱及聚合酶链反应（PCR）指纹图谱类型进行了研究。对27株产毒O1分离株和26株非产毒O1分离株进行BglI酶切后，分别观察到5种和8种图谱类型。使用针对肠杆菌重复基因间共有序列（ERIC）、霍乱弧菌中与ERIC相关的序列、霍乱弧菌中的另一种重复序列（VCR）以及任意序列的3组引物进行PCR指纹图谱分析，分别将相同菌株分为7种和10种PCR类型。8种核糖体分型具有独特的PCR图谱。在其余每种核糖体分型的分离株中，PCR指纹图谱分析均鉴定出不止一种图谱类型。然而，核糖体分型在一个案例中能够区分相同的PCR类型。1993年至1995年间从台湾进口食品及霍乱输入病例中分离出的产毒O1菌株中发现了单一的核糖体分型和单一的PCR图谱类型。通过PCR指纹图谱对霍乱弧菌O1进行分型与核糖体分型相关性良好，但鉴别力更强。PCR检测为这些菌株的分型提供了一种快速简便的方法，用于流行病学研究。
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