用平行快速分析仪微量测定血清钙

Microdetermination of serum calcium by parallel fast analyzer.

作者信息

Dito W R

出版信息

Am J Clin Pathol. 1976 Jun;65(6):1016-21. doi: 10.1093/ajcp/65.6.1016.

DOI:10.1093/ajcp/65.6.1016

Abstract

An adaptation of a direct microdetermination of serum calcium based upon its colored reaction product with cresolphthalein complexone for use with a parallel fast analyzer is described. It is simple, reliable and clinically accurate. Twenty microliters of sample are employed in a final volume of 650 mul. Comparison studies with an atomic absorption method resulted in a correlation coefficinet (r) of 0.94. Replicate analyses at normal (8.5 mg. per dl.) and abnormal (12.1 mg. per dl) levels showed inter-day coefficients of variation of 3.2 and 2.3%, respectively. Assays of sera of male and female donors (age range: 17--60 years) showed normal ranges of 8.5--10.8 and 8.5--10.5 mg. per dl., respectively. Moderate hemolysis and jaundice and mild lipemia are associated with spuriously high results. Magnesium in serum showed no significant effect below 4.0 mEq. per l.

摘要

本文描述了一种基于血清钙与甲酚红络合剂的显色反应产物的直接微量测定法的改进方法，该方法可用于并行快速分析仪。它简单、可靠且临床准确性高。最终体积为650微升时使用20微升样品。与原子吸收法的比较研究得出相关系数（r）为0.94。在正常（每分升8.5毫克）和异常（每分升12.1毫克）水平下的重复分析显示日间变异系数分别为3.2%和2.3%。对男性和女性献血者（年龄范围：17 - 60岁）血清的检测显示正常范围分别为每分升8.5 - 10.8毫克和每分升8.5 - 10.5毫克。中度溶血、黄疸和轻度脂血会导致结果假性升高。血清中镁含量在每升4.0毫当量以下时无显著影响。