Suzuki T, Mitake S, Okumura-Noji K, Yang J P, Fujii T, Okamoto T
Department of Neuroplasticity, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Matsumoto, Japan.
Neuroreport. 1997 Sep 8;8(13):2931-5. doi: 10.1097/00001756-199709080-00025.
The distributions of IkappaB and NF-kappa B immunoreactivities were examined immunohistochemically in the rat brain by the electron microscopy. Antibodies were raised against synthetic peptides with the sequences specific to the human MAD-3 type IkappaB or NF-kappa B. Both IkappaB alpha and NF-kappa B immunoreactivities were localized in the dendrites including the spines and, particularly, the postsynaptic densities (PSDs) of the hippocampus and the cerebral cortex. The PSD fraction prepared from the rat brain contained an activity that inhibited the binding of NF-kappa B to the kappa B DNA elements. These results suggest that the NF-kappa B/IkappaB system or a similar mechanism may play a role in signal transmission from synapses to the nucleus.
通过电子显微镜免疫组织化学方法检测了大鼠脑中IkappaB和NF-kappa B免疫反应性的分布。制备了针对具有人类MAD-3型IkappaB或NF-kappa B特异性序列的合成肽的抗体。IkappaBα和NF-kappa B免疫反应性均定位于包括棘突在内的树突中，特别是海马体和大脑皮质的突触后致密物（PSD）。从大鼠脑中制备的PSD组分含有一种抑制NF-kappa B与kappa B DNA元件结合的活性。这些结果表明，NF-kappa B/IkappaB系统或类似机制可能在从突触到细胞核的信号传递中起作用。
