DiGeronimo M J, Antoine A D
Appl Environ Microbiol. 1976 Jun;31(6):900-6. doi: 10.1128/aem.31.6.900-906.1976.
Six nitrile compounds and two amide derivatives were degraded by Nocardia rhodochrous LL100-21. Acetonitrile, hydroacrylonitrile, and propionitrile were the best sources of carbon and nitrogen for growth, whereas butenenitrile, succinonitrile, and acetamide supported less growth. Acrylonitrile and acrylamide supported growth but only as a source of nitrogen. Gas chromatography of the culture medium revealed a decrease in acetonitrile with the sequential formation of acetamide and acetic acid. Ammonia was also detected by colorimetric procedures. The enzyme system responsible for the hydrolysis of acetonitrile was shown to be intracellular and inducible. The breakdown of acetonitrile by a crude bacterial extract was a two-step enzymatic hydrolysis with acetamide as the intermediate product and acetic acid and ammonia as the final products. Product formation was stoichiometric with substrate disappearance. When propionitrile was the growth substrate, there was complete conversion of the nitrile to propionic acid and ammonia as the major products. The enzymatic breakdown of the propionitrile, although slower than acetonitrile, yielded the corresponding carboxylic acid and ammonia. Propionamide was produced in very small amounts as an intermediate product.
红色诺卡氏菌LL100 - 21可降解六种腈类化合物和两种酰胺衍生物。乙腈、氢氰酸和丙腈是该菌生长的最佳碳源和氮源，而丁烯腈、琥珀腈和乙酰胺支持的生长较少。丙烯腈和丙烯酰胺仅作为氮源支持生长。对培养基进行气相色谱分析发现，乙腈减少，同时依次生成乙酰胺和乙酸。通过比色法也检测到了氨。负责乙腈水解的酶系统显示为胞内酶且可诱导。粗细菌提取物对乙腈的分解是一个两步酶促水解过程，中间产物为乙酰胺，最终产物为乙酸和氨。产物形成与底物消失呈化学计量关系。当丙腈作为生长底物时，腈完全转化为丙酸和氨，这是主要产物。丙腈的酶促分解虽然比乙腈慢，但也产生了相应的羧酸和氨。丙酰胺作为中间产物产生的量非常少。
