自发性高血压大鼠肾髓质磷脂酶C同工酶PLC-δ1的衰减

Attenuation of renomedullary phospholipase C isozyme, PLC-delta 1, in spontaneously hypertensive rats.

作者信息

Lee K H, Cho Y J, Cha S H, Endou H

机构信息

Department of Pharmacology, Catholic University Medical College, Seoul, Korea.

出版信息

Biochem Mol Biol Int. 1997 Nov;43(4):741-7. doi: 10.1080/15216549700204551.

DOI:10.1080/15216549700204551

PMID:9385434

Abstract

The distributional patterns of PLC isozymes within the kidney were investigated using spontaneously hypertensive rats (SHRs) and normotensive Wistar-Kyoto (WKY) rats at 4 and 12 weeks of age. PLC-beta 1, PLC-beta 3 and PLC-delta 1 quantified by Western blot analysis, were present in the highest concentrations in the inner medulla of rats at both 4 and 12 weeks of age. On the other hand, PLC-beta 4, PLC-gamma 1 and PLC-gamma 2 were distributed almost equally among the regions for the rats of both ages. When compared with WKY rats at 12 weeks of age, the amounts of PLC-beta 1, PLC-beta 3, PLC-gamma 1, PLC-gamma 2, and PLC-delta 1 in the inner medulla of SHRs were significantly lower, and the amount of PLC-delta 1 in the inner stripe of the outer medulla was also significantly lower. Even at the prehypertensive stage at 4 weeks of age, the inner medullary concentration of PLC-delta 1 was significantly lower in SHRs than WKY rats. These results suggest that PLC-delta 1 would play an important role in the development of hypertension.

摘要

利用自发性高血压大鼠（SHR）和血压正常的Wistar-Kyoto（WKY）大鼠，在4周龄和12周龄时研究了肾脏内磷脂酶C（PLC）同工酶的分布模式。通过蛋白质印迹分析定量的PLC-β1、PLC-β3和PLC-δ1，在4周龄和12周龄大鼠的内髓质中浓度最高。另一方面，PLC-β4、PLC-γ1和PLC-γ2在两个年龄段大鼠的各区域中分布几乎相等。与12周龄的WKY大鼠相比，SHR内髓质中PLC-β1、PLC-β3、PLC-γ1、PLC-γ2和PLC-δ1的含量显著降低，外髓质内带中PLC-δ1的含量也显著降低。即使在4周龄的高血压前期阶段，SHR内髓质中PLC-δ1的浓度也显著低于WKY大鼠。这些结果表明，PLC-δ1在高血压的发生发展中可能起重要作用。