囊性纤维化患者的不同血清学检查：对铜绿假单胞菌菌体抗原的反应
Different serological examinations in cystic fibrosis patients: response to Pseudomonas aeruginosa St-Ag.
作者信息
Magni A, Trancassini M, Giordano A, De Vito D, Cimino G, Quattrucci S, Antonelli M, Cipriani P
机构信息
Institute of Microbiology, Medical School, La Sapienza University of Rome, Italy.
出版信息
New Microbiol. 1997 Oct;20(4):295-302.
Crossed immunoelectrophoresis (CIE), enzyme linked immunosorbent assay (ELISA) and immunoblotting (IMB) were used to look for the presence of anti-bodies to Pseudomonas aeruginosa standard antigen in the sera of cystic fibrosis patients with intermittent (CF +/- P) and chronic (CF + P) colonization. Our results show that CIE is too complex a method to use when monitoring the infection over time, that with ELISA a correlation with the patient's clinical status may be made and that IMB can evidence an early, albeit low, reaction in the CF +/- P patient.
采用交叉免疫电泳（CIE）、酶联免疫吸附测定（ELISA）和免疫印迹法（IMB），检测间歇性（CF +/- P）和慢性（CF + P）定植的囊性纤维化患者血清中抗铜绿假单胞菌标准抗原抗体的存在情况。我们的结果表明，CIE方法过于复杂，不适用于长期监测感染情况；ELISA方法可与患者的临床状况建立相关性；IMB方法能够证明CF +/- P患者存在早期反应，尽管反应程度较低。
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