通过溴脱氧尿苷光解方法估算紫外线照射的哺乳动物细胞中DNA链延伸速率。
Rate of DNA chain elongation in ultraviolet light-irradiated mammalian cells as estimated by a bromodeoxyuridine photolysis method.
作者信息
Povirk L F, Painter R B
出版信息
Biophys J. 1976 Aug;16(8):883-9. doi: 10.1016/S0006-3495(76)85738-4.
Abstract
Using a new method, we show that, when the DNA chain elongation rate is measured for short time periods, ultraviolet light does not decrease this rate. The method is based on the photolysis of bromouracil-containing DNA by 313 nm light and alkaline sucrose gradients.
摘要
使用一种新方法，我们发现，当在短时间内测量DNA链延伸速率时，紫外线不会降低该速率。该方法基于用313 nm光和碱性蔗糖梯度对含溴尿嘧啶的DNA进行光解。
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2
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Biochim Biophys Acta. 1970 Mar 19;204(1):128-43. doi: 10.1016/0005-2787(70)90496-x.
3
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J Mol Biol. 1969 Nov 14;45(3):467-79. doi: 10.1016/0022-2836(69)90306-4.
4
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