有机溶液、膜以及高效薄层层析板中硫脂的定量测定。

Quantitative determination of thiolipids in organic solution, in membranes, and on HPTLC plates.

作者信息

Zellmer S, Lasch J, Rothe U

机构信息

Martin-Luther-Universität Halle-Wittenberg, Institut für Physiologische Chemie, Halle, Germany.

出版信息

J Lipid Res. 1997 Nov;38(11):2374-9.

PMID:9392435

Abstract

In the presence of ortho-phthalaldehyde and glucosamine, thiolipids form fluorescent isoindole derivatives. This reaction can be used to quantify single- and double-chain mercaptans in membranes (liposomes) and micellar solutions. The lower detection limit is 100 pmol. In addition, the assay allows the detection of 1.9 nmol thiolipids on HPTLC plates and the fluorescence signal is stable for days. A minor modification of the commonly used DTNB (Ellman's) assay allows the quantification of thiolipids in organic solutions at a concentration down to 3 nmol.

摘要

在邻苯二甲醛和氨基葡萄糖存在的情况下，硫脂会形成荧光异吲哚衍生物。该反应可用于定量测定膜（脂质体）和胶束溶液中的单链和双链硫醇。检测下限为100皮摩尔。此外，该测定法可检测高效薄层层析板上的1.9纳摩尔硫脂，且荧光信号可稳定数天。对常用的5,5'-二硫代双(2-硝基苯甲酸)（埃尔曼试剂）测定法稍作修改，即可对浓度低至3纳摩尔的有机溶液中的硫脂进行定量分析。