恰氏疟原虫的糖基磷脂酰肌醇：在啮齿动物模型中研究疟疾糖脂毒素的基础。
Glycosylphosphatidylinositols of Plasmodium chabaudi chabaudi: a basis for the study of malarial glycolipid toxins in a rodent model.
作者信息
Gerold P, Vivas L, Ogun S A, Azzouz N, Brown K N, Holder A A, Schwarz R T
机构信息
Zentrum für Hygiene und Med. Mikrobiologie, Philipps-Universität, Robert-Koch Str. 17, 35037 Marburg, Germany.
出版信息
Biochem J. 1997 Dec 15;328 ( Pt 3)(Pt 3):905-11. doi: 10.1042/bj3280905.
Free and protein-bound glycosylphosphatidylinositols (GPIs) of the blood stages of the rodent malarial parasite Plasmodium chabaudi chabaudi AS were identified and characterized. TLC analysis of material extracted by organic solvents from metabolically labelled parasites revealed a distinct set of glycolipids. These glycolipids were identified as GPIs by specific chemical and enzymic treatments and by structural analysis of their glycan and hydrophobic parts. These analyses revealed that P.c.chabaudi AS synthesizes a set of GPI-biosynthesis intermediates and two potential GPI-anchor precursors exhibiting the following structures: ethanolamine-phosphate [(alpha1-2)mannose]mannose (alpha 1-2) mannose (alpha 1-6) mannose (alpha 1-4) glucosamine - (acyl) inositol-phosphate-diacylglycerol (P.ch. alpha) and ethanolamine-phosphate - mannose (alpha 1-2) mannose (alpha 1-6) mannose (alpha 1-4) glucosamine-(acyl)inositol-phosphate-diacylglycerol (P.ch. beta). One of these GPI-anchor precursors (P.ch. alpha) possesses the same carbohydrate structure as the GPI membrane anchor of merozoite surface protein-1 from P.c.chabaudi AS.
对啮齿类疟原虫恰氏疟原虫AS血液期的游离型和蛋白结合型糖基磷脂酰肌醇（GPI）进行了鉴定和表征。通过有机溶剂从代谢标记的寄生虫中提取的物质进行薄层层析分析，揭示了一组独特的糖脂。通过特定的化学和酶处理以及对其聚糖和疏水部分的结构分析，将这些糖脂鉴定为GPI。这些分析表明，恰氏疟原虫AS合成了一组GPI生物合成中间体和两种潜在的GPI锚定前体，其结构如下：磷酸乙醇胺-[(α1-2)甘露糖]甘露糖(α1-2)甘露糖(α1-6)甘露糖(α1-4)葡糖胺-(酰基)肌醇磷酸-二酰基甘油（恰氏疟原虫α型）和磷酸乙醇胺-甘露糖(α1-2)甘露糖(α1-6)甘露糖(α1-4)葡糖胺-(酰基)肌醇磷酸-二酰基甘油（恰氏疟原虫β型）。其中一种GPI锚定前体（恰氏疟原虫α型）与恰氏疟原虫AS裂殖子表面蛋白-1的GPI膜锚具有相同的碳水化合物结构。
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