使用基质胶培养会抑制小鼠受精卵的发育。
Culture with Matrigel inhibits development of mouse zygotes.
作者信息
Dawson K M, Baltz J M, Claman P
机构信息
Loeb Medical Research Institute, Ottawa Civic Hospital, Ontario, Canada.
出版信息
J Assist Reprod Genet. 1997 Oct;14(9):543-8. doi: 10.1023/a:1021135612118.
PURPOSE
It was reported that Matrigel improved hatching of mouse blastocysts produced in vitro from F1 hybrid-derived zygotes. We investigated whether Matrigel would be similarly beneficial with outbred strain-derived embryos, which exhibit a "two-cell" block similar to the developmental blocks of other species.
METHODS
Mouse embryo development was assessed with or without Matrigel in KSOM medium, which supports the development of blocking strain zygotes in vitro, and in human tubal fluid (HTF) medium, which normally does not but which is used for human IVF.
RESULTS
Matrigel severely inhibited the development of zygotes to blastocysts in KSOM and did not improve culture in HTF. There was no effect on development from the two-cell stage. We were not able to replicate the previous finding of Matrigel's beneficial effect on hatching of F1-derived zygotes.
CONCLUSIONS
Matrigel may be a deleterious addition to embryo culture or coculture systems.
目的
据报道，基质胶可提高由F1杂种来源的受精卵体外产生的小鼠囊胚的孵化率。我们研究了基质胶对远交系来源胚胎是否同样有益，这些胚胎表现出类似于其他物种发育阻滞的“二细胞”阻滞。
方法
在支持阻滞品系受精卵体外发育的KSOM培养基以及通常不支持但用于人类体外受精的人输卵管液（HTF）培养基中，评估有无基质胶时小鼠胚胎的发育情况。
结果
基质胶严重抑制了KSOM中受精卵向囊胚的发育，且未改善HTF中的培养效果。对二细胞阶段的发育没有影响。我们无法重复之前关于基质胶对F1来源受精卵孵化有有益作用的发现。
结论
基质胶可能是胚胎培养或共培养系统中的有害添加物。
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