Delva P, Pastori C, Montesi G, Degan M, Paluani F, Lechi A
Université de Verona, département de clinique médicale, Policlinico Borgo Roma, Verona, Italie.
Arch Mal Coeur Vaiss. 1997 Aug;90(8):1147-9.
To ascertain the claimed links between peripheral insulin resistance and intracellular magnesium and calcium concentrations, we measured free intralymphocyte magnesium (Mg(i)) and calcium (Ca(i)) concentrations, as well as the rate constant of plasma glucose disappearance (K(itt)) after insulin injection (insulin tolerance test: ITT), in a group of 15 normotensive control subjects (NC) and 29 essential hypertensive subjects (EH). Mg(i) and Ca(i) were measured in triplicate by means of a fluorimetric technique based on the dyes furaptra and fura-2 respectively. K(itt) value were significantly reduced in hypertensive subjects as compared to control subjects (M +/- SD, EH: 4.54 +/- 1.31 vs 5.63 +/- 1.07; p < 0.02; 95% confidence limits: 0.22-1.96). Mg(i) and Ca(i) were not statistically different in hypertensive subjects as compared to control subjects (Mg(i), NC: 0.274 +/- 0.02 mmol/L; EH: 0.248 +/- 0.05 mmol/L; Ca(i), NC: 47.6 +/- 9 mmol/L, EH: 46.7 +/- 13.6 mmol/L). A statistically significant inverse correlation was found in the whole study group between K(itt) and body mass index (R = -0.394, p = 0.01) and a statistically significant positive correlation between K(itt) and Mg(i) (R = 0.386; p = 0.012). The latter correlation was no longer statistically significant if adjusted for body mass index. Our data are in favour of a link between index of peripheral insulin resistance and body mass index. A dependence from Mg(i) is possible but the study lack so far the statistical power to demonstrate it.
为了确定外周胰岛素抵抗与细胞内镁和钙浓度之间所宣称的联系，我们在15名血压正常的对照受试者（NC）和29名原发性高血压受试者（EH）中，测量了淋巴细胞内游离镁（Mg(i)）和钙（Ca(i)）浓度，以及注射胰岛素后血浆葡萄糖消失速率常数（K(itt)）（胰岛素耐量试验：ITT）。分别采用基于染料氟普塔和fura-2的荧光技术对Mg(i)和Ca(i)进行了三次测量。与对照受试者相比，高血压受试者的K(itt)值显著降低（均值±标准差，EH：4.54±1.31，NC：5.63±1.07；p<0.02；95%置信区间：0.22 - 1.96）。与对照受试者相比，高血压受试者的Mg(i)和Ca(i)无统计学差异（Mg(i)，NC：0.274±0.02 mmol/L；EH：0.248±0.05 mmol/L；Ca(i)，NC：47.6±9 mmol/L，EH：46.7±13.6 mmol/L）。在整个研究组中，发现K(itt)与体重指数之间存在统计学显著的负相关（R = -0.394，p = 0.01），K(itt)与Mg(i)之间存在统计学显著的正相关（R = 0.386；p = 0.012）。如果对体重指数进行校正，后一种相关性不再具有统计学显著性。我们的数据支持外周胰岛素抵抗指数与体重指数之间存在联系。可能依赖于Mg(i)，但目前该研究缺乏证明这一点的统计效力。
