使用引物特异性聚合酶链反应快速检测腐生葡萄球菌

Rapid detection of Staphylococcus saprophyticus using primer specific PCR.

作者信息

Gaszewska-Mastalarz A, Zakrzewska-Czerwinska J, Mordarski M

机构信息

Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.

出版信息

Acta Biol Hung. 1997;48(3):319-22.

PMID:9406611

Abstract

Staphylococcus saprophyticus is one of the most frequently encountered clinically significant members of the coagulase-negative staphylococci. A set of species-specific PCR primers was defined for the detection of Staphylococcus saprophyticus. These primers target variable regions (V3 and V6) of the 16S rRNA gene. Primer-specific PCR has potential applications in epidemiological studies and diagnosis of Staphylococcus saprophyticus.

摘要

腐生葡萄球菌是凝固酶阴性葡萄球菌中临床上最常遇到的具有重要意义的成员之一。已定义了一组物种特异性PCR引物用于检测腐生葡萄球菌。这些引物靶向16S rRNA基因的可变区（V3和V6）。引物特异性PCR在腐生葡萄球菌的流行病学研究和诊断中具有潜在应用。