抗坏血酸盐刺激人眼球筋膜成纤维细胞中的I型和III型胶原蛋白。
Ascorbate stimulates type I and type III collagen in human Tenon's fibroblasts.
作者信息
Wendt M D, Soparkar C N, Louie K, Basinger S F, Gross R L
机构信息
Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, TX 77030, USA.
出版信息
J Glaucoma. 1997 Dec;6(6):402-7.
PURPOSE
To better understand wound healing after glaucoma filtration surgery by measuring the production of type I and type III collagen in cultured Tenon's fibroblasts and determine the effect of ascorbic acid on collagen subtype production.
METHODS
An ELISA-type dot blot assay was used to directly measure the production of types I and III collagen by subconfluent cultures of fibroblasts from human Tenon's capsule. Because ascorbic acid is both high in aqueous humor and necessary for the production of collagen, we measured the dose response of type I and type III collagen production to ascorbic acid.
RESULTS
Ascorbic acid stimulated an increase in collagen production that reached a maximum level at 100 micrograms/ml. This is approximately half of the ascorbic acid concentration found in human aqueous humor. Unlike previous reports, we found no toxic effects from ascorbic acid at concentrations as high as 250 micrograms/ml over a 24-hour period. The lack of toxicity may result from the use of serum-free media in the assay.
CONCLUSIONS
This culture system will be useful for exploring factors that may alter collagen production and could potentially affect wound healing.
目的
通过测量培养的提上睑肌腱膜成纤维细胞中I型和III型胶原蛋白的产生，更好地了解青光眼滤过术后的伤口愈合情况，并确定抗坏血酸对胶原蛋白亚型产生的影响。
方法
采用酶联免疫吸附试验（ELISA）斑点印迹法直接测量人提上睑肌腱膜成纤维细胞亚汇合培养物中I型和III型胶原蛋白的产生。由于房水中抗坏血酸含量高且是胶原蛋白产生所必需的，我们测量了I型和III型胶原蛋白产生对抗坏血酸的剂量反应。
结果
抗坏血酸刺激胶原蛋白产生增加，在100微克/毫升时达到最高水平。这大约是人房水中抗坏血酸浓度的一半。与先前的报道不同，我们发现在24小时内，高达250微克/毫升的抗坏血酸没有毒性作用。缺乏毒性可能是由于试验中使用了无血清培养基。
结论
该培养系统将有助于探索可能改变胶原蛋白产生并可能影响伤口愈合的因素。
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