Jung H C, Mun K H, Park T C, Lee Y C, Park J M, Huh K, Seong D H, Suh J K
Department of Urology, School of Medicine, College of Pharmacy, Yeungnam University, Taegu, Korea.
Yonsei Med J. 1997 Oct;38(5):261-9. doi: 10.3349/ymj.1997.38.5.261.
The present study was undertaken to investigate the role of nitric oxide (NO) in erectile physiology by correlating its action with the existence and activity of nitric oxide synthase (NOS), which produces NO. We applied Western blot analysis in both human and rat penile tissue. In the rat, reduced nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase staining and spectrophotometric assay were also performed, in addition to in vivo electroerection study with pharmacological manipulation. Western blot analysis identified a protein of 155 KDa identical to the neural form of NOS in the human and rat penis. The NOS blot densities in the two species were similar, and both were lower than that in the rat cerebellum. Histochemical staining localized NOS to neurons innervating the corpora cavernosa, including the pelvic plexus, the cavernosal nerves and their terminal fibers within the corporeal erectile tissue, and dorsal penile nerves. NOS activity was also found in the cerebellum, urethra, penis, and urinary bladder, in decreasing order of intensity. Intracavernous injections of NOS inhibitor (L-NOARG or L-NAME in concentrations from 10(-6) M to 10(-3) M suppressed electrostimulation-induced erection in a concentration-dependent manner. Subsequent intracavernous injection of L-Arginine (10(-2) M) partially restored the erection. The neural form of constitutive NOS in the corpora cavernosa synthesizes NO, which mediates penile erection. Determination of cavernosal NOS expression or activity may permit characterization of certain pathological conditions that cause impotence.
本研究旨在通过将一氧化氮（NO）的作用与产生NO的一氧化氮合酶（NOS）的存在及活性相关联，来探讨NO在勃起生理中的作用。我们对人类和大鼠的阴茎组织进行了蛋白质印迹分析。对于大鼠，除了进行药理学操作的体内电勃起研究外，还进行了还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）黄递酶染色和分光光度测定。蛋白质印迹分析在人类和大鼠阴茎中鉴定出一种与神经型NOS相同的155 kDa蛋白质。两个物种中的NOS印迹密度相似，且均低于大鼠小脑的印迹密度。组织化学染色将NOS定位到支配海绵体的神经元，包括盆腔丛、海绵体神经及其在海绵体勃起组织内的终末纤维，以及阴茎背神经。在小脑、尿道、阴茎和膀胱中也发现了NOS活性，强度依次降低。海绵体内注射NOS抑制剂（L - NOARG或L - NAME，浓度为10^(-6) M至10^(-3) M）以浓度依赖的方式抑制电刺激诱导的勃起。随后海绵体内注射L - 精氨酸（10^(-2) M）可部分恢复勃起。海绵体中组成型NOS的神经型合成NO，其介导阴茎勃起。测定海绵体NOS的表达或活性可能有助于对某些导致阳痿的病理状况进行特征描述。
