冬季比目鱼肾脏有机阴离子转运体的表达克隆与特性分析
Expression cloning and characterization of a renal organic anion transporter from winter flounder.
作者信息
Wolff N A, Werner A, Burkhardt S, Burckhardt G
机构信息
Abteilung Vegetative Physiologie, Zentrum Physiologie und Pathophysiologie, Georg-August-Universität Göttingen, Germany.
出版信息
FEBS Lett. 1997 Nov 17;417(3):287-91. doi: 10.1016/s0014-5793(97)01304-5.
The cDNA coding for a renal p-aminohippurate (PAH) transporter from winter flounder (Pseudopleuronectes americanus), designated fROAT, was cloned by functional expression in Xenopus laevis oocytes. fROAT is approximately 2.8 kbp in length and encodes a protein of 562 amino acids, related to the rat renal organic anion transporter ROAT1/OAT1 and the organic cation transporters OCT1 and OCT2. In oocytes, fROAT mediated probenecid-sensitive PAH uptake, with a Km for PAH of about 20 microM, and inhibited by external glutarate (GA) (1 mM). The functional characteristics suggest that fROAT is the basolateral PAH/dicarboxylate exchanger of the flounder kidney.
通过在非洲爪蟾卵母细胞中的功能表达，克隆出了编码冬比目鱼（美洲拟庸鲽）肾脏对氨基马尿酸（PAH）转运体的互补DNA（cDNA），命名为fROAT。fROAT长度约为2.8千碱基对，编码一个由562个氨基酸组成的蛋白质，与大鼠肾脏有机阴离子转运体ROAT1/OAT1以及有机阳离子转运体OCT1和OCT2相关。在卵母细胞中，fROAT介导丙磺舒敏感的PAH摄取，PAH的米氏常数约为20微摩尔，且受到细胞外戊二酸（GA）（1毫摩尔）的抑制。这些功能特性表明fROAT是比目鱼肾脏基底外侧的PAH/二羧酸交换体。
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