来自茶树叶子的碳酸酐酶。

Carbonic anhydrase from Camelia sinensis (tea) leaves.

作者信息

Demir Y, Demir N, Ağar G

机构信息

Atatürk Universitesi Eğitim Fakültesi Kimya Eğitimi Bölümü.

出版信息

Prep Biochem Biotechnol. 1997 Nov;27(4):271-8. doi: 10.1080/10826069708001284.

DOI:10.1080/10826069708001284

PMID:9413559

Abstract

Carbonic anhydrase (CA) (carbonate hydrolyase; E,C,4.2.1.1) from leaves of mature Camelia sinensis was purified and characterized. The purification level was 53 fold. The optimum temperature for maximal enzyme activity is 50 degrees C. The optimum pH was 6.8 and this pH varied between 6.5 and 7.5. Each enzyme molecule is a hexamer with an M(r) of 169,000 with subunits of M(r) = 28,000.

摘要

对成熟茶树叶片中的碳酸酐酶（CA）（碳酸水解酶；E.C.4.2.1.1）进行了纯化和特性鉴定。纯化倍数为53倍。酶活性达到最大值时的最适温度为50℃。最适pH为6.8，该pH在6.5至7.5之间变化。每个酶分子是一个六聚体，相对分子质量为169,000，亚基相对分子质量为28,000。