Pex19p，一种对过氧化物酶体生物发生至关重要的法尼基化蛋白。
Pex19p, a farnesylated protein essential for peroxisome biogenesis.
作者信息
Götte K, Girzalsky W, Linkert M, Baumgart E, Kammerer S, Kunau W H, Erdmann R
机构信息
Institut für Physiologische Chemie, Ruhr-Universität Bochum, Germany.
出版信息
Mol Cell Biol. 1998 Jan;18(1):616-28. doi: 10.1128/MCB.18.1.616.
Abstract
We report the identification and molecular characterization of Pex19p, an oleic acid-inducible, farnesylated protein of 39.7 kDa that is essential for peroxisome biogenesis in Saccharomyces cerevisiae. Cells lacking Pex19p are characterized by the absence of morphologically detectable peroxisomes and mislocalization of peroxisomal matrix proteins to the cytosol. The human HK33 gene product was identified as the putative human ortholog of Pex19p. Evidence is provided that farnesylation of Pex19p takes place at the cysteine of the C-terminal CKQQ amino acid sequence. Farnesylation of Pex19p was shown to be essential for the proper function of the protein in peroxisome biogenesis. Pex19p was shown to interact with Pex3p in vivo, and this interaction required farnesylation of Pex19p.
摘要
我们报告了Pex19p的鉴定和分子特征，它是一种39.7 kDa的油酸诱导型、法尼基化蛋白，对酿酒酵母中的过氧化物酶体生物发生至关重要。缺乏Pex19p的细胞的特征是没有形态上可检测到的过氧化物酶体，并且过氧化物酶体基质蛋白错误定位于细胞质中。人类HK33基因产物被鉴定为Pex19p的推定人类同源物。有证据表明，Pex19p的法尼基化发生在C末端CKQQ氨基酸序列的半胱氨酸处。Pex19p的法尼基化被证明对该蛋白在过氧化物酶体生物发生中的正常功能至关重要。Pex19p在体内被证明与Pex3p相互作用，并且这种相互作用需要Pex19p的法尼基化。
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Peroxisomes: Organelles at the crossroads.过氧化物酶体：处于交汇点的细胞器。
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Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways.Pex14p，一种过氧化物酶体膜蛋白，结合两种依赖于过氧化物酶体靶向信号（PTS）的导入途径的受体。
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PEX genes on the rise.PEX基因正在增加。
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Multiple PEX genes are required for proper subcellular distribution and stability of Pex5p, the PTS1 receptor: evidence that PTS1 protein import is mediated by a cycling receptor.过氧化物酶体生物发生因子（PEX）基因中的多个基因对于PTS1受体Pex5p的正确亚细胞分布和稳定性是必需的：这表明PTS1蛋白的导入是由循环受体介导的。
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Proteins involved in peroxisome biogenesis and functioning.参与过氧化物酶体生物发生和功能的蛋白质。
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Protein translocation into peroxisomes.蛋白质转运到过氧化物酶体中。
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Identification of Pex13p a peroxisomal membrane receptor for the PTS1 recognition factor.过氧化物酶体基质靶向信号1（PTS1）识别因子的过氧化物酶体膜受体Pex13p的鉴定。
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The SH3 domain of the Saccharomyces cerevisiae peroxisomal membrane protein Pex13p functions as a docking site for Pex5p, a mobile receptor for the import PTS1-containing proteins.酿酒酵母过氧化物酶体膜蛋白Pex13p的SH3结构域作为Pex5p的停靠位点，Pex5p是一种用于导入含PTS1蛋白的移动受体。
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10
Pex13p is an SH3 protein of the peroxisome membrane and a docking factor for the predominantly cytoplasmic PTs1 receptor.Pex13p是一种过氧化物酶体膜的SH3蛋白，也是主要位于细胞质中的PTs1受体的对接因子。
J Cell Biol. 1996 Oct;135(1):85-95. doi: 10.1083/jcb.135.1.85.
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