P1 RepA与质粒Rts1复制起点的相互作用：起始蛋白诱导外源起点进行复制的能力。

Interaction of P1 RepA with replication origin of plasmid Rts1: capability of an initiator protein inducing replication from a foreign origin.

作者信息

Li Y F, Tabuchi A, Terawaki Y

机构信息

Department of Bacteriology, Shinshu University School of Medicine, Matsumoto, Japan.

出版信息

Biochem Biophys Res Commun. 1997 Dec 18;241(2):570-3. doi: 10.1006/bbrc.1997.7859.

DOI:10.1006/bbrc.1997.7859

PMID:9425312

Abstract

Rts1 RepA and P1 RepA are trans-acting proteins essential for the initiation of replication of plasmid Rts1 and prophage P1, respectively. In this study, we found that, in vitro, P1 RepA bound to the Rts1 ori fragment and Rts1 incI fragment as strongly as Rts1 RepA. In addition P1 RepA, in trans, activated the Rts1 replication origin that was cloned in pBR322, thus allowing the ori plasmid to be maintained in a polA E. coli host. Under these conditions, however, the ori plasmid was unstable as compared with that when activated by Rts1 RepA. In addition, we found that Rts1 RepA showed no interaction with the P1 replication origin.

摘要

Rts1 RepA和P1 RepA分别是质粒Rts1和原噬菌体P1复制起始所必需的反式作用蛋白。在本研究中，我们发现，在体外，P1 RepA与Rts1 ori片段和Rts1 incI片段的结合强度与Rts1 RepA相同。此外，P1 RepA可反式激活克隆于pBR322的Rts1复制起点，从而使ori质粒能在polA大肠杆菌宿主中得以维持。然而，在这些条件下，与被Rts1 RepA激活时相比，ori质粒不稳定。另外，我们发现Rts1 RepA与P1复制起点没有相互作用。