使用微透析技术监测清醒大鼠心脏的交感和副交感神经支配。

Use of microdialysis for monitoring sympathetic and parasympathetic innervation of heart in conscious rats.

作者信息

Cremers T I, Teisman A C, van Gilst W H, Westerink B H

机构信息

Department of Medicinal Chemistry, Faculty of Medicine, University of Groningen, The Netherlands.

出版信息

Am J Physiol. 1997 Dec;273(6):H2850-6. doi: 10.1152/ajpheart.1997.273.6.H2850.

DOI:10.1152/ajpheart.1997.273.6.H2850

PMID:9435623

Abstract

A microdialysis method was developed to sample norepinephrine and acetylcholine from the heart of freely moving rats. A flexible dialysis fiber (length 14 mm), with a copper wire inserted inside, was implanted into the heart. Extracellular norepinephrine was detectable for at least 72 h after implantation. Basal output levels 24 h after surgery were 140 pg/ml when corrected for in vitro recovery. Evidence was provided that the major part of norepinephrine in dialysates is derived from local neurotransmission. Acetylcholine was only detectable in cardiac dialysates when an esterase inhibitor was infused. Corrected basal output levels 24 h after surgery were 223 pg/ml when neostigmine was coinfused in a concentration of 100 mumol/l. In addition, the presence of local muscarinic autoreceptors on cholinergic neurons in the heart was shown. It is concluded that microdialysis is a reliable method that can be used to study the innervation of the heart in subchronic preparations in freely moving rats.

摘要

开发了一种微透析方法，用于从自由活动大鼠的心脏中采集去甲肾上腺素和乙酰胆碱。将一根内部插入铜丝的柔性透析纤维（长度14毫米）植入心脏。植入后至少72小时可检测到细胞外去甲肾上腺素。术后24小时经体外回收率校正后的基础输出水平为140 pg/ml。有证据表明，透析液中去甲肾上腺素的主要部分来源于局部神经传递。仅在注入酯酶抑制剂时，心脏透析液中才可检测到乙酰胆碱。当以100 μmol/l的浓度共同注入新斯的明时，术后24小时经校正的基础输出水平为223 pg/ml。此外，还显示了心脏胆碱能神经元上存在局部毒蕈碱自受体。得出的结论是，微透析是一种可靠的方法，可用于研究自由活动大鼠亚慢性制剂中心脏的神经支配。