Analysis of mitomycin C-induced micronuclei in lymphocytes from malnourished infected children.

The purpose of this study was to determine if peripheral blood lymphocytes from malnourished children with gastrointestinal or respiratory bacterial infection show increased frequencies of Mitomycin C (MMC)-induced micronuclei as compared to well-nourished, infected children. The results indicate that cells from malnourished, infected children had greater chromosome damage. This may indicate that such children would be more susceptible to environmental damage and malignant transformation. Micronucleus frequencies were analyzed in binucleate cells produced by the cytokinesis block method; the overall micronucleus frequency was significantly higher in binucleate cells from malnourished, infected children. The mean micronucleus frequency in MMC-free cultures was 4.3/1000 in malnourished infected children and 1.0/1000 in well-nourished infected children. In MMC-exposed cultures the mean induced micronucleus frequency was 32.6 +/- 6.1 vs. 12.9 +/- 2.3; 68.6 +/- 12.1 vs. 21.0 +/- 5.1, and 88.1 +/- 16.2 vs. 41.7 +/- 5.0 for malnourished and well-nourished children at 20, 40, and 60 ng/ml MMC, respectively. The number of binucleated cells with more than one micronucleus was also higher in malnourished, infected children at all doses tested, including cells with two micronuclei in MMC-free cultures from malnourished, infected children. This increase was not found in peripheral blood lymphocytes from well-nourished infected children.