小鼠细胞周期蛋白G1基因的基因组结构与染色体定位

Genomic structure and chromosomal localization of mouse cyclin G1 gene.

作者信息

Kimura S H, Kataoka T R, Endo Y, Nojima H

机构信息

Department of Molecular Genetics, Osaka University, Japan.

出版信息

Genomics. 1997 Dec 15;46(3):483-6. doi: 10.1006/geno.1997.5034.

DOI:10.1006/geno.1997.5034

PMID:9441755

Abstract

Mouse genomic DNA harboring the full coding sequence of cyclin G1 was cloned and analyzed. The locations of five coding exons and the intron-exon boundary sequences were found to be conserved between the mouse and the human genes. Two putative binding sites for the p53 tumor suppressor gene product were found around the first exon: one was located in the 5' regulatory region, and the other was in the first intron. The mouse cyclin G1 gene was mapped to bands A5 to B1 of chromosomes 11 (11A5-B1) by FISH using genomic DNA clone as a biotinylated probe. The location of mouse cyclin G1 is syntenic to that of its human homologue, which we previously mapped to 5q32-q34 of chromosome 5. An additional faint signal was detected on chromosome 4 (4B1-C2), probably indicating the presence of a cyclin G1-related gene or pseudogene in the mouse genome.

摘要

克隆并分析了携带细胞周期蛋白G1完整编码序列的小鼠基因组DNA。发现小鼠和人类基因之间五个编码外显子的位置以及内含子-外显子边界序列是保守的。在第一个外显子周围发现了两个假定的p53肿瘤抑制基因产物结合位点：一个位于5'调控区，另一个位于第一个内含子中。使用基因组DNA克隆作为生物素化探针，通过荧光原位杂交（FISH）将小鼠细胞周期蛋白G1基因定位到11号染色体的A5至B1带（11A5-B1）。小鼠细胞周期蛋白G1的位置与其人类同源物的位置是同线的，我们之前将其人类同源物定位到5号染色体的5q32-q34。在4号染色体（4B1-C2）上检测到一个额外的微弱信号，可能表明小鼠基因组中存在细胞周期蛋白G1相关基因或假基因。