从杆状病毒启动子表达的冈比亚按蚊色氨酸加氧酶基因可互补黑腹果蝇朱红基因。

The Anopheles gambiae tryptophan oxygenase gene expressed from a baculovirus promoter complements Drosophila melanogaster vermilion.

作者信息

Besansky N J, Mukabayire O, Benedict M Q, Rafferty C S, Hamm D M, Mcnitt L

机构信息

Department of Biological Sciences, University of Notre Dame, IN 46556, USA.

出版信息

Insect Biochem Mol Biol. 1997 Aug-Sep;27(8-9):803-5. doi: 10.1016/s0965-1748(97)00040-4.

DOI:10.1016/s0965-1748(97)00040-4

PMID:9443379

Abstract

An Anopheles gambiae cDNA encoding tryptophan oxygenase was placed under the control of the constitutive baculovirus promoter, ie-1. The chimeric construct, expressed transiently in vermilion (tryptophan oxygenase) mutants of Drosophila melanogaster, partially rescued adult eye color. The successful genetic complementation by this construct demonstrated both the proper function of the tryptophan oxygenase product and the effectiveness of the ie-1 promoter in directing expression of foreign genes in live insects. The functionality of An. gambiae tryptophan oxygenase in a higher fly fulfils predictions based on its structural conservation throughout millions of years of independent evolution.

摘要

将编码色氨酸加氧酶的冈比亚按蚊cDNA置于组成型杆状病毒启动子ie-1的控制之下。该嵌合构建体在黑腹果蝇的朱红色（色氨酸加氧酶）突变体中瞬时表达，部分挽救了成虫的眼色。该构建体成功的遗传互补证明了色氨酸加氧酶产物的正常功能以及ie-1启动子在指导外源基因在活昆虫中表达方面的有效性。冈比亚按蚊色氨酸加氧酶在高等果蝇中的功能符合基于其在数百万年独立进化过程中的结构保守性所做的预测。