Jan C R, Chen W C, Lee Y H, Huang J K, Ou H C, Tseng C J
Department of Medical Education and Research, Veterans General Hospital-Kaohsiung, Taiwan, Republic of China.
Urol Res. 1997;25(6):427-32. doi: 10.1007/BF01268861.
Allopurinol has been reported to ameliorate the side effects in patients following shock wave lithotripsy (SWL); however, the mechanism has not been studied. We have examined the protective effect of allopurinol on Madin-Darby canine kidney (MDCK) cells after shock wave exposure (SWE) by determining the release of aspartate aminotransferase (ASAT) and lactate dehydrogenase (LD), and the resting cytosolic Ca2+ concentration ([Ca2+]i). In SWE-treated cells, the release of ASAT and LD increased immediately, but largely transiently, by approximately 23% and 5-fold over control, respectively. Within 1-6 h after SWE there was a gradual rise in the resting [Ca2+]i of 16-137% above control. Allopurinol did not affect the transient enzyme release but blocked the long-term rises in the resting [Ca2+]i. The transient changes in [Ca2+]i evoked by two hormones, ATP and bradykinin, and a drug that releases Ca2+ from internal Ca2+ stores, thapsigargin, were only slightly affected in allopurinol-treated cells. We conclude that the protection conferred by allopurinol on patients treated with SWL might involve a direct protection of the kidney cells by maintaining a normal resting [Ca2+]i.
据报道，别嘌醇可改善冲击波碎石术（SWL）后患者的副作用；然而，其机制尚未得到研究。我们通过测定天冬氨酸转氨酶（ASAT）和乳酸脱氢酶（LD）的释放以及静息胞质Ca2+浓度（[Ca2+]i），研究了别嘌醇对冲击波暴露（SWE）后犬肾Madin-Darby（MDCK）细胞的保护作用。在SWE处理的细胞中，ASAT和LD的释放立即增加，但大多是短暂的，分别比对照增加约23%和5倍。在SWE后1-6小时内，静息[Ca2+]i逐渐升高，比对照高16%-137%。别嘌醇不影响短暂的酶释放，但阻止了静息[Ca2+]i的长期升高。在别嘌醇处理的细胞中，由两种激素ATP和缓激肽以及一种从细胞内Ca2+储存中释放Ca2+的药物毒胡萝卜素引起的[Ca2+]i的短暂变化仅受到轻微影响。我们得出结论，别嘌醇对接受SWL治疗的患者的保护作用可能涉及通过维持正常的静息[Ca2+]i对肾细胞的直接保护。
