莫洛尼鼠白血病病毒整合酶蛋白的一个中心半胱氨酸残基和HHCC结构域在功能性多聚化中的作用。
Implication of a central cysteine residue and the HHCC domain of Moloney murine leukemia virus integrase protein in functional multimerization.
作者信息
Donzella G A, Leon O, Roth M J
机构信息
Department of Biochemistry, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854, USA.
出版信息
J Virol. 1998 Feb;72(2):1691-8. doi: 10.1128/JVI.72.2.1691-1698.1998.
Abstract
Moloney murine leukemia virus (M-MuLV) IN-IN protein interactions important for catalysis of strand transfer and unimolecular and bimolecular disintegration reactions were investigated by using a panel of chemically modified M-MuLV IN proteins. Functional complementation of an HHCC-deleted protein (Ndelta105) by an independent HHCC domain (Cdelta232) was severely compromised by NEM modification of either subunit. Productive Ndelta105 IN-DNA interactions with a disintegration substrate lacking a long terminal repeat 5'-single-stranded tail also required complementation by a functional HHCC domain. Virus encoding the C209A M-MuLV IN mutation exhibited delayed virion production and replication kinetics.
摘要
通过使用一组化学修饰的莫洛尼鼠白血病病毒（M-MuLV）整合酶（IN）蛋白，研究了对链转移催化以及单分子和双分子解体反应至关重要的M-MuLV IN-IN蛋白相互作用。独立的HHCC结构域（Cdelta232）对HHCC缺失蛋白（Ndelta105）的功能互补，因任一亚基的NEM修饰而严重受损。Ndelta105 IN与缺乏长末端重复序列5'-单链尾巴的解体底物之间有效的相互作用也需要功能性HHCC结构域的互补。编码C209A M-MuLV IN突变的病毒表现出延迟的病毒粒子产生和复制动力学。
相似文献
1
Implication of a central cysteine residue and the HHCC domain of Moloney murine leukemia virus integrase protein in functional multimerization.莫洛尼鼠白血病病毒整合酶蛋白的一个中心半胱氨酸残基和HHCC结构域在功能性多聚化中的作用。
J Virol. 1998 Feb;72(2):1691-8. doi: 10.1128/JVI.72.2.1691-1698.1998.
2
Coordinated disintegration reactions mediated by Moloney murine leukemia virus integrase.由莫洛尼鼠白血病病毒整合酶介导的协同解体反应。
J Virol. 1996 Jun;70(6):3909-21. doi: 10.1128/JVI.70.6.3909-3921.1996.
3
Functional domains of Moloney murine leukemia virus integrase defined by mutation and complementation analysis.通过突变和互补分析确定的莫洛尼鼠白血病病毒整合酶的功能结构域
J Virol. 1996 Jul;70(7):4585-97. doi: 10.1128/JVI.70.7.4585-4597.1996.
4
Functional interactions of the HHCC domain of moloney murine leukemia virus integrase revealed by nonoverlapping complementation and zinc-dependent dimerization.通过非重叠互补和锌依赖性二聚化揭示莫洛尼鼠白血病病毒整合酶HHCC结构域的功能相互作用。
J Virol. 1999 Mar;73(3):1809-17. doi: 10.1128/JVI.73.3.1809-1817.1999.
5
Mutational analysis of the N-terminus of Moloney murine leukemia virus integrase.莫洛尼鼠白血病病毒整合酶N端的突变分析
Virology. 2001 Dec 5;291(1):32-45. doi: 10.1006/viro.2001.1218.
6
Role of the His-Cys finger of Moloney murine leukemia virus integrase protein in integration and disintegration.莫洛尼鼠白血病病毒整合酶蛋白的组氨酸-半胱氨酸指在整合和解离中的作用。
J Virol. 1993 Sep;67(9):5562-71. doi: 10.1128/JVI.67.9.5562-5571.1993.
7
X-ray crystal structure of the N-terminal region of Moloney murine leukemia virus integrase and its implications for viral DNA recognition.莫洛尼鼠白血病病毒整合酶N端区域的X射线晶体结构及其对病毒DNA识别的意义。
Proteins. 2017 Apr;85(4):647-656. doi: 10.1002/prot.25245. Epub 2017 Feb 3.
8
Assembly and catalysis of concerted two-end integration events by Moloney murine leukemia virus integrase.莫洛尼鼠白血病病毒整合酶对协同性两端整合事件的组装与催化作用。
J Virol. 2001 Oct;75(20):9561-70. doi: 10.1128/JVI.75.20.9561-9570.2001.
9
Differential multimerization of Moloney murine leukemia virus integrase purified under nondenaturing conditions.在非变性条件下纯化的莫洛尼鼠白血病病毒整合酶的差异多聚化。
Virology. 2003 Nov 10;316(1):146-60. doi: 10.1016/s0042-6822(03)00559-2.
10
Revealing domain structure through linker-scanning analysis of the murine leukemia virus (MuLV) RNase H and MuLV and human immunodeficiency virus type 1 integrase proteins.通过对鼠白血病病毒（MuLV）核糖核酸酶H以及MuLV和1型人类免疫缺陷病毒整合酶蛋白进行接头扫描分析来揭示结构域结构。
J Virol. 2006 Oct;80(19):9497-510. doi: 10.1128/JVI.00856-06.
引用本文的文献
1
Crosslinking and mass spectrometry suggest that the isolated NTD domain dimer of Moloney murine leukemia virus integrase adopts a parallel arrangement in solution.交联和质谱分析表明，莫洛尼鼠白血病病毒整合酶的分离的NTD结构域二聚体在溶液中呈平行排列。
BMC Struct Biol. 2013 Jul 11;13:14. doi: 10.1186/1472-6807-13-14.
2
Blue native PAGE and biomolecular complementation reveal a tetrameric or higher-order oligomer organization of the physiological measles virus attachment protein H.蓝色 native PAGE 和生物分子互补实验揭示了麻疹病毒附着蛋白 H 的四聚体或更高阶寡聚体的生理结构。
J Virol. 2010 Dec;84(23):12174-84. doi: 10.1128/JVI.01222-10. Epub 2010 Sep 22.
3
Differential multimerization of Moloney murine leukemia virus integrase purified under nondenaturing conditions.在非变性条件下纯化的莫洛尼鼠白血病病毒整合酶的差异多聚化。
Virology. 2003 Nov 10;316(1):146-60. doi: 10.1016/s0042-6822(03)00559-2.
4
Assembly and catalysis of concerted two-end integration events by Moloney murine leukemia virus integrase.莫洛尼鼠白血病病毒整合酶对协同性两端整合事件的组装与催化作用。
J Virol. 2001 Oct;75(20):9561-70. doi: 10.1128/JVI.75.20.9561-9570.2001.
5
Dissecting the role of the N-terminal domain of human immunodeficiency virus integrase by trans-complementation analysis.通过反式互补分析剖析人类免疫缺陷病毒整合酶N端结构域的作用。
J Virol. 1999 Apr;73(4):3176-83. doi: 10.1128/JVI.73.4.3176-3183.1999.
6
Functional interactions of the HHCC domain of moloney murine leukemia virus integrase revealed by nonoverlapping complementation and zinc-dependent dimerization.通过非重叠互补和锌依赖性二聚化揭示莫洛尼鼠白血病病毒整合酶HHCC结构域的功能相互作用。
J Virol. 1999 Mar;73(3):1809-17. doi: 10.1128/JVI.73.3.1809-1817.1999.
本文引用的文献
1
Solution structure of the N-terminal zinc binding domain of HIV-1 integrase.人类免疫缺陷病毒1型整合酶N端锌结合结构域的溶液结构
Nat Struct Biol. 1997 Jul;4(7):567-77. doi: 10.1038/nsb0797-567.
2
Zinc folds the N-terminal domain of HIV-1 integrase, promotes multimerization, and enhances catalytic activity.锌可折叠HIV-1整合酶的N端结构域，促进多聚化，并增强催化活性。
Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13659-64. doi: 10.1073/pnas.93.24.13659.
3
Retroviral integrase, putting the pieces together.逆转录病毒整合酶：整合过程解析
J Biol Chem. 1996 Aug 16;271(33):19633-6. doi: 10.1074/jbc.271.33.19633.
4
Functional domains of Moloney murine leukemia virus integrase defined by mutation and complementation analysis.通过突变和互补分析确定的莫洛尼鼠白血病病毒整合酶的功能结构域
J Virol. 1996 Jul;70(7):4585-97. doi: 10.1128/JVI.70.7.4585-4597.1996.
5
Coordinated disintegration reactions mediated by Moloney murine leukemia virus integrase.由莫洛尼鼠白血病病毒整合酶介导的协同解体反应。
J Virol. 1996 Jun;70(6):3909-21. doi: 10.1128/JVI.70.6.3909-3921.1996.
6
The metal ion-induced cooperative binding of HIV-1 integrase to DNA exhibits a marked preference for Mn(II) rather than Mg(II).金属离子诱导的HIV-1整合酶与DNA的协同结合对Mn(II)表现出明显的偏好，而非Mg(II)。
J Biol Chem. 1996 Jan 19;271(3):1498-506. doi: 10.1074/jbc.271.3.1498.
7
Human immunodeficiency virus type 1 integrase mutants retain in vitro integrase activity yet fail to integrate viral DNA efficiently during infection.1型人类免疫缺陷病毒整合酶突变体在体外仍保留整合酶活性，但在感染过程中不能有效地整合病毒DNA。
J Virol. 1996 Feb;70(2):721-8. doi: 10.1128/JVI.70.2.721-728.1996.
8
Identification of the catalytic and DNA-binding region of the human immunodeficiency virus type I integrase protein.人免疫缺陷病毒I型整合酶蛋白催化及DNA结合区域的鉴定
Nucleic Acids Res. 1993 Mar 25;21(6):1419-25. doi: 10.1093/nar/21.6.1419.
9
Characterization of human immunodeficiency virus type 1 integrase expressed in Escherichia coli and analysis of variants with amino-terminal mutations.在大肠杆菌中表达的1型人类免疫缺陷病毒整合酶的特性鉴定及氨基末端突变变体分析。
J Virol. 1993 Jan;67(1):425-37. doi: 10.1128/JVI.67.1.425-437.1993.
10
Role of the His-Cys finger of Moloney murine leukemia virus integrase protein in integration and disintegration.莫洛尼鼠白血病病毒整合酶蛋白的组氨酸-半胱氨酸指在整合和解离中的作用。
J Virol. 1993 Sep;67(9):5562-71. doi: 10.1128/JVI.67.9.5562-5571.1993.
Zotero 插件