冷冻保存的未成熟小鼠卵母细胞：一项染色体和纺锤体研究。

Cryopreserved immature mouse oocytes: a chromosomal and spindle study.

作者信息

Frydman N, Selva J, Bergere M, Auroux M, Maro B

机构信息

Reproductive Biology and Cytogenetics Laboratory, Hospital Kremlin Bicêtre, France.

出版信息

J Assist Reprod Genet. 1997 Nov;14(10):617-23. doi: 10.1023/a:1022593004528.

DOI:10.1023/a:1022593004528

PMID:9447465

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3454737/

Abstract

PURPOSE

Cryopreservation of human oocytes might provide an alternative approach to freezing supernumerary embryos obtained during IVF. This process, performed on immature denuded prophase 1 mouse oocytes, was investigated.

METHODS

We first investigated the capacity of frozen, immature, murine oocytes to continue in vitro maturation after thawing. We then evaluated the risk to offspring from chromosomal damage by cytogenetical and cytological (spindle) analysis. Finally, we attempted to determine the reasons for and the stage of maturation failure.

RESULTS

A total of 700 immature oocytes was frozen, 629 (90%) were recovered intact after thawing, and 53% extruded the first polar body, versus 74% for the control group. Freezing was not accompanied by an increase in aneuploidy in maturing oocytes (18 and 15% for thawed and control oocytes, respectively). Consequently, the first meiotic division occurred normally, without an increase in nondisjunction. Spindle analysis demonstrated only a few abnormalities (15 and 2% for thawed and control oocytes, respectively) incompatible with further development. Oocytes arrested during in vitro maturation were mainly at the metaphase I stage (64 and 76% for thawed and control oocytes, respectively). Whereas 17% of thawed oocytes were blocked before the formation of the first meiotic spindle, this never occurred in the control group.

CONCLUSIONS

Immature murine oocytes can withstand cryo-preservation, which is encouraging for future human application of this technique.

摘要

目的

人类卵母细胞的冷冻保存可能为体外受精过程中获得的多余胚胎冷冻提供一种替代方法。本研究对未成熟的裸前期1小鼠卵母细胞进行了该过程的研究。

方法

我们首先研究了冷冻的未成熟小鼠卵母细胞解冻后继续体外成熟的能力。然后，通过细胞遗传学和细胞学（纺锤体）分析评估了染色体损伤对后代的风险。最后，我们试图确定成熟失败的原因和阶段。

结果

总共冷冻了700个未成熟卵母细胞，解冻后629个（90%）完整回收，53%的卵母细胞排出了第一极体，而对照组为74%。冷冻并未导致成熟卵母细胞非整倍体增加（解冻卵母细胞和对照卵母细胞分别为18%和15%）。因此，第一次减数分裂正常发生，不分离现象没有增加。纺锤体分析仅显示少数异常（解冻卵母细胞和对照卵母细胞分别为15%和2%），这些异常与进一步发育不相容。体外成熟过程中停滞的卵母细胞主要处于中期I阶段（解冻卵母细胞和对照卵母细胞分别为64%和76%）。虽然17%的解冻卵母细胞在第一个减数分裂纺锤体形成之前受阻，但对照组从未出现这种情况。