从荧光威克汉姆酵母中分离并鉴定一种新的双链RNA病毒
Isolation and characterization of a new dsRNA virus from Wickerhamia fluorescens.
作者信息
Pospísek M, Palková Z, Korb J, Vanĕk D
机构信息
Department of Genetics and Microbiology, Charles University, Prague, Czech Republic.
出版信息
Folia Microbiol (Praha). 1996;41(3):223-7. doi: 10.1007/BF02814620.
PMID:9449770
Abstract
Virus-like particles (VLPs) were isolated from the yeast Wickerhamia fluorescens strain CCY61-1-1. The VLPs are approximately 42 nm in diameter and contain only one species of dsRNA molecule. The apparent length of the dsRNA determined by native agarose gel electrophoresis was 4.6 kbp. Analysis of protein content of the VLPs showed them to contain one major capsid protein with an apparent molar mass of 74.5 kDa.
摘要
从荧光威克汉姆酵母菌株CCY61-1-1中分离出病毒样颗粒（VLPs）。这些VLPs直径约为42纳米，仅包含一种双链RNA分子。通过天然琼脂糖凝胶电泳测定的双链RNA的表观长度为4.6千碱基对。对VLPs蛋白质含量的分析表明，它们含有一种主要衣壳蛋白，表观摩尔质量为74.5千道尔顿。
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