Kagayama M, Zhu J X, Sasano Y, Sato H, Mayanagi H
Department of Anatomy, Tohoku University School of Dentistry, Sendai, Japan.
Anat Embryol (Berl). 1997 Dec;196(6):477-83. doi: 10.1007/s004290050115.
The development of interglobular dentine in the first upper and lower molars of Wistar rats aged 3, 7, 14, 21, 42 days was examined histochemically using a lectin, succinyl wheat germ agglutinin (sWGA), which is specific for N-acetyl-D-glucosamine. sWGA stained the interglobular dentine, predentin and Golgi area of odontoblasts. Interglobular dentine was not formed in the first molars of 3-day rats, but appeared in those of 7-day rats near the enamel-free area. In 14-day rats, interglobular dentine was present in most areas of the coronal dentine except the cervical area. At the interface between dentine and predentin, numerous sWGA-negative calcospherites were seen, suggesting that the interglobular dentine is formed actively there. In 21-day rats, the interlobular dentine was more numerous than in 14-day rats. Interglobular dentine was present in the cervical root dentine as well as in the coronal dentine, including the cervical area. The distribution of interglobular dentine in 42-day rats was similar to that in 21-day rats, but fluorescence of sWGA binding was less intense in the former. Because the development of interglobular dentine appeared to be time and position specific its relation to the stages of ameloblasts was analysed. Thin enamel matrix was formed at cusps in molars of 3-day rats and thickness of enamel matrix increased in 7-day rats. In these teeth, the ameloblasts were at the differentiating or secretory stage. The Golgi area and Tomes' processes of the secretory ameloblasts, the cells of intermediate layer and the enamel matrix were weakly positive with sWGA. The epithelial cells at the enamel-free area were also stained with sWGA. In 14-day rats, most of the ameloblasts in the first maxillary molars were at the maturative stage except in the cervical area, where the ameloblasts were at the transitional stage. sWGA stained the distal border and the Golgi area of the maturative ameloblasts as well as the cells of the papillary layer. The distal border of the maturative ameloblasts appeared either thick or thin, suggesting a ruffle-end and smooth-end of the cells. Ameloblasts were absent in the first molars of 21-day rats and the cervical part of the enamel was covered with the stratified epithelium like that of 42-day rats. The present study has demonstrated that interglobular dentine contains sWGA-binding glycoconjugates and the formation of the interglobular dentine is largely associated with the enamel maturation. These results suggest that matrix-to-cell interaction is important for the development of interglobular dentine.
采用对N - 乙酰 - D - 葡糖胺具有特异性的凝集素——琥珀酰小麦胚凝集素（sWGA），对3、7、14、21、42日龄Wistar大鼠的第一上下磨牙球间牙本质的发育进行了组织化学检查。sWGA对球间牙本质、前期牙本质和成牙本质细胞的高尔基体区域进行了染色。3日龄大鼠的第一磨牙未形成球间牙本质，但在7日龄大鼠无釉质区域附近出现了球间牙本质。在14日龄大鼠中，除颈部区域外，冠状牙本质的大部分区域都存在球间牙本质。在牙本质与前期牙本质的界面处，可见大量sWGA阴性的钙化球，提示球间牙本质在此处活跃形成。在21日龄大鼠中，球间牙本质比14日龄大鼠更多。球间牙本质存在于颈部根牙本质以及包括颈部区域在内的冠状牙本质中。42日龄大鼠球间牙本质的分布与21日龄大鼠相似，但前者sWGA结合荧光强度较弱。由于球间牙本质的发育似乎具有时间和位置特异性，因此分析了其与成釉细胞阶段的关系。3日龄大鼠磨牙的牙尖处形成了薄的釉基质，7日龄大鼠釉基质厚度增加。在这些牙齿中，成釉细胞处于分化或分泌阶段。分泌期成釉细胞、中间层细胞和釉基质的高尔基体区域及托姆斯突与sWGA呈弱阳性。无釉质区域的上皮细胞也被sWGA染色。在14日龄大鼠中，第一上颌磨牙的大多数成釉细胞处于成熟阶段，颈部区域除外，该区域的成釉细胞处于过渡阶段。sWGA对成熟成釉细胞的远端边界和高尔基体区域以及乳头层细胞进行了染色。成熟成釉细胞的远端边界看起来要么厚要么薄，提示细胞的皱褶端和平滑端。21日龄大鼠的第一磨牙中无成釉细胞，釉质的颈部部分被分层上皮覆盖，与42日龄大鼠相似。本研究表明，球间牙本质含有sWGA结合糖缀合物，球间牙本质的形成在很大程度上与釉质成熟相关。这些结果表明，基质与细胞的相互作用对球间牙本质的发育很重要。
