In situ hybridization on blood smears for diagnosis of chicken anemia virus in broiler breeder flocks.

Chicken anemia virus (CAV) infection was suspected in progeny from broiler breeder hens raised in a location that was geographically different from where most pullets were raised for one Georgia production company. These breeder pullets were raised in new or relatively new houses. Progeny from these pullets experienced a 10-fold increase in average daily mortality over progeny from flocks raised in established breeder houses. Lesions in the broilers included severe necrotizing dermatitis, pale bone marrow, and small thymus glands. Infection with CAV was confirmed in the breeders by enzyme-linked immunosorbent assay, indirect immunofluorescence, and in situ hybridization on peripheral blood smears. The in situ hybridization identified three broiler breeder flocks that were actively infected with CAV and were probably the source of infection in broiler flocks.