血栓素合成酶抑制剂对大鼠原位肝移植中无心跳供体的影响。

Effect of thromboxane synthetase inhibitor on non-heart-beating donors in rat orthotopic liver transplantation.

作者信息

Soejima Y, Yanaga K, Nisizaki T, Yoshizumi T, Uchiyama H, Sugimachi K

机构信息

Department of Surgery II, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

出版信息

Surgery. 1998 Jan;123(1):67-72.

PMID:9457225

Abstract

BACKGROUND

The use of non-heart-beating donors could relieve donor organ shortage provided that the outcome of the recipients is not jeopardized.

METHODS

In this study we evaluated the effect of thromboxane synthetase inhibitor (OKY-046) on non-heart-beating donors in orthotopic liver transplantation in rodents. OKY-046 (OKY group, n = 8) or vehicle (control group, n = 8) was given to the donor animals for 60 minutes before the induction of 30 minutes of warm ischemia to the liver before transplantation and survival, and several parameters were compared between the two groups.

RESULTS

All recipients in the control group died within 2 days, whereas 67% of those of the OKY group had a 2-day survival and 44% had a 2-week survival (p < 0.01). Bile output from the allograft for 2 hours after reperfusion was significantly larger in the OKY group at all time points (p < 0.05). Furthermore, serum transaminase levels 10 minutes after reperfusion were significantly lower in the OKY group (p < 0.05), although those 120 minutes after reperfusion were comparable between the two groups. The thromboxane B2 level in liver tissue 10 and 120 minutes after reperfusion was significantly lower in the OKY group compared with the control group (p < 0.02 and p < 0.05, respectively).

CONCLUSIONS

These results indicate that OKY-046 given to the donor before the induction of warm ischemia could ameliorate ischemia/reperfusion injury of the hepatic allograft by reducing thromboxane A2 production after reperfusion.

摘要

背景

在不危及受者预后的前提下，使用非心脏跳动供体可缓解供体器官短缺的问题。

方法

在本研究中，我们评估了血栓素合成酶抑制剂（OKY-046）对啮齿动物原位肝移植中非心脏跳动供体的影响。在对供体动物进行移植前肝脏30分钟热缺血诱导及观察存活情况前60分钟，给予OKY-046（OKY组，n = 8）或赋形剂（对照组，n = 8），并比较两组的几个参数。

结果

对照组所有受者均在2天内死亡，而OKY组67%的受者存活了2天，44%的受者存活了2周（p < 0.01）。再灌注后2小时内，OKY组在所有时间点的移植肝胆汁分泌量均显著更高（p < 0.05）。此外，再灌注10分钟后OKY组的血清转氨酶水平显著更低（p < 0.05），尽管再灌注120分钟后两组相当。与对照组相比，OKY组再灌注10分钟和120分钟时肝组织中的血栓素B2水平显著更低（分别为p < 0.02和p < 0.05）。