甘氨酸通过EDTA增强对大鼠肝脏核苷酸焦磷酸酶/磷酸二酯酶-I的抑制作用：对酸性成纤维细胞生长因子（FGF-1）商业制剂所报道的抑制作用的全面阐述。

Glycine-enhanced inhibition of rat liver nucleotide pyrophosphatase/phosphodiesterase-I by EDTA: a full account of the reported inhibition by commercial preparations of acidic fibroblast growth factor (FGF-1).

作者信息

López-Gómez J, Costas M J, Meireles Ribeiro J, Fernández A, Romero A, Avalos M, Cameselle J C

机构信息

Unidad de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Extremadura, Badajoz, Spain.

出版信息

FEBS Lett. 1998 Jan 2;421(1):77-9. doi: 10.1016/s0014-5793(97)01536-6.

DOI:10.1016/s0014-5793(97)01536-6

PMID:9462844

Abstract

The earlier reported inhibition of rat liver nucleotide pyrophosphatase/phosphodiesterase I (EC 3.1.6.9/EC 3.1.4.1; NPP/PDE) by culture-grade acidic fibroblast growth factor (FGF-1) correlates with a low-Mr contaminant. 1H-NMR analyses revealed EDTA in the total-volume fractions of a gel-filtration experiment, where all the inhibitory activity of the FGF-1 preparation was recovered. NPP/PDE inhibition by EDTA (and by unfractionated FGF-1 or the EDTA-containing fractions) was time-dependent, blocked by the substrate p-nitrophenyl-dTMP, and strongly enhanced by glycine. The use of glycine buffers in earlier work was critical to the apparent inhibition by FGF-1. The results point to a conformational change favored by glycine that may be relevant to the biological role of NPP/PDE.

摘要

先前报道的培养级酸性成纤维细胞生长因子（FGF-1）对大鼠肝脏核苷酸焦磷酸酶/磷酸二酯酶I（EC 3.1.6.9/EC 3.1.4.1；NPP/PDE）的抑制作用与一种低分子量污染物有关。1H-NMR分析在凝胶过滤实验的总体积组分中检测到了EDTA，FGF-1制剂的所有抑制活性在这些组分中得以恢复。EDTA（以及未分级的FGF-1或含EDTA的组分）对NPP/PDE的抑制作用具有时间依赖性，可被底物对硝基苯基-dTMP阻断，并被甘氨酸强烈增强。早期研究中使用甘氨酸缓冲液对于FGF-1的明显抑制作用至关重要。结果表明，甘氨酸有利于一种构象变化，这可能与NPP/PDE的生物学作用相关。